揭开心脏隐藏敌人的面纱:基于心脏类器官芯片对聚苯乙烯纳米塑料诱发心脏毒性的动态观察

IF 15.8 1区 材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY
ACS Nano Pub Date : 2024-11-12 Epub Date: 2024-11-01 DOI:10.1021/acsnano.4c13262
Tianyi Zhang, Sheng Yang, Yiling Ge, Lihong Yin, Yuepu Pu, Zhongze Gu, Zaozao Chen, Geyu Liang
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引用次数: 0

摘要

接触微塑料和纳米塑料(MNPs)可能会引起心脏毒性。然而,基于心肌细胞系的体外模型缺乏关键的心脏特征,而动物模型的种间差异也影响了结论的可靠性。此外,目前的研究主要集中于 MNPs 的单时点暴露,忽视了对早期和晚期心脏损伤的比较分析。此外,在了解病理条件下对 MNPs 的易感性方面仍存在很大差距。针对这些局限性,本研究整合了心脏器官组织(COs)和芯片上器官(OoC)技术,开发了芯片上心脏器官组织(COoC),并从多个维度验证了其在心脏毒性评价中的有效性。基于 COoC,我们对短期和长期暴露于聚苯乙烯纳米塑料(PS-NPs)造成的心脏损伤进行了动态观察。氧化应激、炎症、钙离子平衡破坏和线粒体功能障碍被证实是 PS-NP 诱导心脏毒性的潜在机制,也是早期阶段的关键事件,而心脏纤维化则是晚期阶段的突出特征。值得注意的是,尽管在健康模型中未显示出明显的心脏毒性,但在病理状态下,低剂量暴露会加重心肌梗死症状。总之,这些发现进一步加深了我们对 PS-NP 诱导的心脏毒性效应的理解,并为评估心脏毒性引入了一个前景广阔的体外平台。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Unveiling the Heart's Hidden Enemy: Dynamic Insights into Polystyrene Nanoplastic-Induced Cardiotoxicity Based on Cardiac Organoid-on-a-Chip.

Unveiling the Heart's Hidden Enemy: Dynamic Insights into Polystyrene Nanoplastic-Induced Cardiotoxicity Based on Cardiac Organoid-on-a-Chip.

Exposure to micro- and nanoplastics (MNPs) has been implicated in potential cardiotoxicity. However, in vitro models based on cardiomyocyte cell lines lack crucial cardiac characteristics, while interspecies differences in animal models compromise the reliability of the conclusions. In addition, current research has predominantly focused on single-time point exposures to MNPs, neglecting comparative analyses of cardiac injury across early and late stages. Moreover, there remains a large gap in understanding the susceptibility to MNPs under pathological conditions. To address these limitations, this study integrated cardiac organoids (COs) and organ-on-a-chip (OoC) technology to develop the cardiac organoid-on-a-chip (COoC), which was validated for cardiotoxicity evaluation through multiple dimensions. Based on COoC, we conducted a dynamic observation of the cardiac damage caused by short- and long-term exposure to polystyrene nanoplastics (PS-NPs). Oxidative stress, inflammation, disruption of calcium ion homeostasis, and mitochondrial dysfunction were confirmed as the potential mechanisms of PS-NP-induced cardiotoxicity and the crucial events in the early stages, while cardiac fibrosis emerged as a prominent feature in late stages. Notably, low-dose exposure exacerbated myocardial infarction symptoms under pathological states, despite no significant cardiotoxicity shown in healthy models. In conclusion, these findings further deepened our understanding of PS-NP-induced cardiotoxic effects and introduced a promising in vitro platform for assessing cardiotoxicity.

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来源期刊
ACS Nano
ACS Nano 工程技术-材料科学:综合
CiteScore
26.00
自引率
4.10%
发文量
1627
审稿时长
1.7 months
期刊介绍: ACS Nano, published monthly, serves as an international forum for comprehensive articles on nanoscience and nanotechnology research at the intersections of chemistry, biology, materials science, physics, and engineering. The journal fosters communication among scientists in these communities, facilitating collaboration, new research opportunities, and advancements through discoveries. ACS Nano covers synthesis, assembly, characterization, theory, and simulation of nanostructures, nanobiotechnology, nanofabrication, methods and tools for nanoscience and nanotechnology, and self- and directed-assembly. Alongside original research articles, it offers thorough reviews, perspectives on cutting-edge research, and discussions envisioning the future of nanoscience and nanotechnology.
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