从临床阴性分离株中发现的具有多重耐药性的铜绿假单胞菌携带的 IncP6 质粒上的 qnrVC1 基因的基因组学启示

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology Pub Date : 2024-11-01 DOI:10.1016/j.vetmic.2024.110285

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引用次数: 0

摘要

铜绿假单胞菌（P. aeruginosa）是一种机会性病原体，由于其抗菌药耐药性而对全球健康造成严重威胁。在马匹中，铜绿假单胞菌可引起感染，尤其是生殖道感染，导致繁殖失败。耐多药（MDR）铜绿假单胞菌一直是包括驴业在内的畜牧业关注的焦点。本研究旨在阐明从中国河北省一个大型集约化养殖场中患有子宫内膜炎的驴身上分离出的铜绿假单胞菌株的系统发育关系。通过全基因组测序预测了编码多种抗菌素耐药性的基因。多焦点序列分型（MLST）显示，所有菌株都属于同一序列类型（ST1058）。鉴定出了编码 qnrVC1 基因的 IncP6 质粒，该质粒与喹诺酮类药物耐药性有关。基因组比较分析表明了菌株的特征和 qnrVC1 的遗传背景。本研究首次报道了这些 MDR 铜绿微囊桿菌asinine 菌株表现出了由携带 qnrVC1 的质粒赋予的高水平抗菌药和金属抗性。此外，还发现了整合了巨型质粒的铜绿假单胞菌菌株。从 "一体健康 "的角度来看，该研究强调了监测包括驴在内的食用动物抗菌药耐药性基因的重要性。

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Genomic insights into qnrVC1 gene located on an IncP6 plasmid carried by multidrug resistant Pseudomonas aeruginosa from clinical asinine isolates

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen, causing significant global health threat due to its antimicrobial resistance. Among equines, P. aeruginosa can cause infections, particularly in the reproductive tract, leading to reproductive failure. Multidrug-resistant (MDR) P. aeruginosa has been a major concern in animal husbandry, including the donkey industry. The study aims to elucidate the phylogenetic relationship of P. aeruginosa strains isolated from donkeys with endometritis farmed in a large intensive unit in Hebei Province, China. Genes coding for multiple antimicrobial resistances were predicted by whole genomic sequencing. Multilocus sequence typing (MLST) revealed that all strains belonged to the same sequence type (ST1058). An IncP6 plasmid encoding the qnrVC1 gene, associated with quinolone resistance, was identified. Comparative genomic analysis illustrated the characteristics of the strains and genetic context of qnrVC1. This study is the first to report that these MDR P. aeruginosa asinine strains exhibited high levels of antimicrobial and metal resistance conferred by a qnrVC1-carrying plasmid. Additionally, P. aeruginosa strains with integrated mega-plasmids were identified. From a One Health perspective, the study underlined the significance of monitoring antimicrobial resistance genes in food animals, including donkeys.

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.