[使用β-内酰胺酶与特异性抗体共轭的免疫酶法测定铜绿假单胞菌外毒素A]。

Antibiotiki i meditsinskaia biotekhnologiia = Antibiotics and medical biotechnology Pub Date : 1987-09-01

I A Aleksandrova, A Iu Sazykin, A D Aleksandrov, A F Moroz

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摘要

用亲和层析法在外毒素 Aepharose 4B 上分离出用于铜绿假单胞菌外毒素 A 免疫酶法检测的特异性抗体。通过连接戊二醛，制备了外毒素 A 抗体与地衣芽孢杆菌 749/c 的β-内酰胺酶的共轭物。该共轭物具有很高的免疫特异性和酶活性，可用于竞争性固相免疫酶法检测。该检测方法灵敏度高，可测定 20 纳克/毫升浓度的外毒素 A。它可用于测定培养液中的铜绿假单胞菌外毒素 A。

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[Immunoenzyme method of determining Pseudomonas aeruginosa exotoxin A by using a conjugate of beta-lactamase with specific antibodies].

Specific antibodies for immunoenzymatic assay of P. aeruginosa exotoxin A were isolated with affinity chromatography on exotoxin A-Sepharose 4B. A conjugate of the antibodies to exotoxin A with beta-lactamase from Bacillus licheniformis 749/c was prepared by linking glutaric aldehydes. The conjugate showed high immunospecific and enzymatic activity and was used for competitive solid phase immunoenzymatic assay. Sensitivity of the assay provided determination of exotoxin A in concentration of 20 ng/ml. It was used for determining P. aeruginosa exotoxin A in culture fluids.

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Antibiotiki i meditsinskaia biotekhnologiia = Antibiotics and medical biotechnology

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