关于 miR-29a 通过下调 TAGLN2 抑制甲状腺乳头状癌的研究

IF 1.7 4区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES

Journal of Radiation Research and Applied Sciences Pub Date : 2024-10-22 DOI:10.1016/j.jrras.2024.101155

Yibing Wang , Qiuting Wen , Xiangguo Jin , Ming Tao , Yanbin Xu , Yulou Wang , Liran Cui , Xingjiang Li , Feng Zhang

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引用次数: 0

摘要

目的本研究旨在探讨 miR-29a 通过下调 TAGLN2 抑制甲状腺乳头状癌（PTC）的可能机制。方法分别用 MTS 试验、伤口愈合试验和 Matrigel 基质侵袭试验检测 PTC 细胞生长、迁移和侵袭能力的变化。用 Western 印迹法测定 E-cadherin、N-cadherin、vimentin 和 MMP2 的表达。比较不同组间蛋白质表达的差异。结果 miR-29a 能显著抑制 K1 细胞的增殖、迁移、侵袭和上皮-间质转化（EMT）。通过调节肌动蛋白结合蛋白TAGLN2的表达，miR-29a的过表达在改善PTC恶性表型方面发挥了重要作用。TAGLN2的过表达可以抵消miR-29a对K1细胞的抑制作用。结论 miR-29a/TAGLN2 轴可能通过调节下游 PI3K/AKT 信号通路影响 K1 细胞的恶性表型，从而为治疗 PTC 提供了一个新靶点。

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Study on miR-29a inhibiting papillary thyroid carcinoma by downregulating TAGLN2

Objective

This study aims to explore the possible mechanism of miR-29a inhibiting papillary thyroid carcinoma (PTC) through the downregulation of TAGLN2. The paper expects to find novel targets for PTC treatment and prognosis improvement, and is also of great significance in enhancing the overall therapeutic level of PTC and improving life quality of patients.

Methods

Changes in PTC cell growth, migration, and invasive ability were respectively detected using MTS assays, wound healing experiments, and Matrigel matrix invasion assays. Expressions of E-cadherin, N-cadherin, vimentin, and MMP2 were determined by Western blotting. Differences in protein expressions between groups were compared. Overexpression and knockdown of TAGLN2 were performed in K1 cells, and the expression levels of PI3K/AKT pathway protein in K1 cells were detected by Western blotting.

Result

miR-29a significantly inhibited the proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of K1 cells. Overexpression of miR-29a played an important role in improving the malignant phenotype of PTC by regulating the expression of actin-binding protein TAGLN2. Overexpression of TAGLN2 can counteract the inhibitory effect of miR-29a on K1 cells. TAGLN2 can activate the PI3K/AKT signaling pathway, indicating that it may promote the progress of EMT by activating PI3K/AKT.

Conclusion

The miR-29a/TAGLN2 axis may affect the malignant phenotype of K1 cells by regulating downstream PI3K/AKT signaling pathways, thus providing a new target for the treatment of PTC.

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来源期刊

Journal of Radiation Research and Applied Sciences MULTIDISCIPLINARY SCIENCES-

自引率

5.90%

发文量

130

审稿时长

16 weeks

期刊介绍： Journal of Radiation Research and Applied Sciences provides a high quality medium for the publication of substantial, original and scientific and technological papers on the development and applications of nuclear, radiation and isotopes in biology, medicine, drugs, biochemistry, microbiology, agriculture, entomology, food technology, chemistry, physics, solid states, engineering, environmental and applied sciences.