Peperomia pellucida (L.) Kunth 对牙周炎大鼠的抗炎功效

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Dewi Lidya Ichwana Nasution , Sri Tjahajawati , Ratna Indriyanti , Amaliya Amaliya
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引用次数: 0

摘要

背景牙周炎以牙周袋深度(PPD)为特征,有利于细菌定植和炎症,因此需要辅助治疗。虽然 0.2 % 洗必泰(CHX)漱口水很有效,但其副作用导致人们开始寻找替代疗法。本研究旨在探讨 Peperomia pellucida (L.) Kunth 提取物对诱导性牙周炎大鼠的抗炎功效。采用四组 Wistar 株 Rattus norvegicus 大鼠:原始组(无牙周炎)、阴性对照组(仅诱发牙周炎)、阳性对照组(诱发牙周炎并注射 0.2 % CHX)和实验组(诱发牙周炎并注射 2.5 μL 胡椒萃取物)。每个治疗组每天给药一周。在第 0、3、5 和 7 天测量 PPD。第 7 天采集血清进行 ELISA 检测,以测定 IL-1β、TNF-α、IL-10 和 IL-13 的水平。结果经提取物处理的大鼠 PPD 有所下降,提取物组与阴性对照组之间存在显著差异(p < 0.05)。提取物组的 TNF-α 水平与阴性对照组有显著差异(p < 0.05),但与原始对照组和阳性对照组无显著差异。IL-1β 水平仅与阴性对照组有显著差异。IL-10水平与原始组和阴性对照组均有显著差异,而IL-13水平仅与阴性对照组有显著差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti-inflammatory effectiveness of Peperomia pellucida (L.) Kunth in rats induced with periodontitis

Background

Periodontitis, marked by deep periodontal pocket depth (PPD), facilitates bacterial colonization and inflammation, necessitating adjunctive therapies. Although 0.2 % chlorhexidine (CHX) mouthwash is effective, its side effects have led to the search for alternative treatments. Peperomia pellucida (L.) Kunth, known as Pepper Elder, is a traditional medicinal plant with potential as an adjunctive herbal therapy for periodontitis.

Objective

This study aimed to investigate the anti-inflammatory efficacy of Peperomia pellucida extract in rats with induced periodontitis.

Methods

A post-test control group design was used in this laboratory experimental study. Four groups of Wistar strain Rattus norvegicus rats were utilized: a Pristine group (without periodontitis), a negative control group (induced periodontitis only), a positive control group (induced periodontitis and administered 0.2 % CHX), and an experimental group (induced periodontitis and administered 2.5 μL of Pepper Elder extract). Each treatment group received daily administration for one week. PPD measurements were taken on days 0, 3, 5, and 7. Blood serum was collected on day 7 for ELISA to measure IL-1β, TNF-α, IL-10, and IL-13 levels. Statistical analysis was performed using the Kruskal-Wallis test with a post hoc LSD and Mann-Whitney test.

Results

The extract-treated rats showed a decrease in PPD, with significant differences between the extract group and the negative control group (p < 0.05). TNF-α levels in the extract group differed significantly from the negative control group (p < 0.05) but not from the Pristine and positive control groups. IL-1β levels differed significantly only from the negative control group. IL-10 levels were significantly different from both the Pristine and negative control groups, while IL-13 levels differed significantly only from the negative control group.

Conclusion

Peperomia pellucida (L.) Kunth extract exhibits anti-inflammatory effects in rats with induced periodontitis.
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来源期刊
Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
4.60
自引率
0.00%
发文量
191
审稿时长
59 days
期刊介绍: Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.
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