马来西亚半岛反刍动物养殖场中的蜱和蜱传病原体：Rhipicephalus microplus（Acari：Ixodidae）中 Borrelia theileri 的首个分子证据。

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2024-10-22 DOI:10.1016/j.vprsr.2024.101145

Abdul Rahman Kazim , Van Lun Low , Jamal Houssaini , Dennis Tappe , Chong Chin Heo

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引用次数: 0

摘要

为了揭示蜱传疾病的重要性，尤其是经常与农场工人接触的农场动物中的蜱传疾病，本研究旨在确定马来西亚反刍动物中的蜱和蜱传病原体。因此，研究人员在马来西亚半岛各地进行了标本采集，从 674 头农场反刍动物身上共采集到 1241 只蜱虫。在这些蜱虫中，确定了 4 个蜱虫物种，其中以 Rhipicephalus microplus 最为普遍，占蜱虫总数的 99.03%。对 130 个蜱池的分析显示，有 3 个蜱池的包柔氏病毒呈阳性。对 flaB 和 16S rRNA 基因的 BLAST 分析表明，它们与 Theileri Borrelia 的相似度很高，flaB 为 98.78%-100%，16S rRNA 为 99.23%-99.45%。这些结果与系统发生树一致，在系统发生树中，两个基因的序列都与鲍氏不动杆菌聚类在一起，进一步支持了这一鉴定。没有检测到立克次体和巴顿氏菌。这项研究是马来西亚首次在小加鼠体内发现 B. theileri。

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Ticks and tick-borne pathogens in ruminant farms of Peninsular Malaysia: First molecular evidence of Borrelia theileri in Rhipicephalus microplus (Acari: Ixodidae).

To shed light on the importance of tick-borne diseases, especially in farm animals that often contact with farm workers, this study aimed to identify ticks and tick-borne pathogens in ruminants in Malaysia. Accordingly, specimen collection was conducted across Peninsular Malaysia yielded a total of 1241 ticks collected from 674 farm ruminants. Among these, four tick species were identified, with Rhipicephalus microplus being the most prevalent, constituting 99.03 % of the total tick population. Analysis of 130 tick pools revealed three positives for Borrelia. BLAST analyses of the flaB and 16S rRNA genes revealed high similarities to Borrelia theileri, ranging from 98.78 to 100 % for flaB and 99.23–99.45 % for 16S rRNA. These results align with the phylogenetic trees, where sequences from both genes clustered together with B. theileri, further supporting this identification. No Rickettsia and Bartonella bacteria were detected. This study represents the first occurrence of B. theileri in R. microplus in Malaysia.

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).