Live Cell Imaging Studies on Orange Emitting Thiazole-Pyridone Azo Fluorophore and Its Latent Fingerprints, Computational, Electrochemical Sensing for Dopamine Detection

The fluorescent materials have sparked a lot of research interests due to their unique electronic, optical and chemical characteristics. Here, we are intended to present a simple and facile synthesis of novel orange emitting thiazole-pyridone fluorescent tag (TPFT) by a simple diazo coupling reaction and the structural elucidation was carried out by IR, NMR (¹H and ¹³C), UV–Vis, photoluminescence and HR-MS spectrometry. The solvatochromic behaviour of the TPFT offered crucial information about the formation of hydrazone and azo tautomeric forms. The DFT simulations are computed to calculate HOMO-LUMO energy gap (3.028 eV) of TPFT along with MEP and RDG analyses. Comprehensive LFP visualization is revealed under both normal and UV light conditions (365 nm). The cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used to analyse the electrochemical behaviour of the TPFT-modified glassy carbon electrode (MGCE) and exhibited a lower detection limit of 7.89 × 10⁻⁸ M (S/N = 3) with a linear range of 0.5–8.0 μM for DA detection. The live-cell imaging study of TPFT showed a strong blue emission at 453 nm, which generally indicates the existence of fluorescence stability.