人体骨骼肌外周耦合中 DHPR 四元组的老化相关结构研究。

IF 1.8 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Laura Pietrangelo, Rosa Mancinelli, Stefania Fulle, Simona Boncompagni
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引用次数: 0

摘要

在骨骼肌衰老过程中发生的众多变化中,受伤后再生潜力降低的主要原因是卫星细胞增殖和分化能力受损。在此,我们利用冷冻断裂电子显微镜技术,分析了年轻受试者(28 岁)的肌管在分化 9 天后和老年受试者(71 岁)的肌管在分化 9 天和 12 天后的二氢吡啶受体(DHPRs)四联体(4 个颗粒)的发生率和大小。与年轻肌管相比,在分化 9 天时,老年肌管表现出:i) 较低的 DHPR 群发生率和较小的 DHPR 群大小;ii) 较低的完整四聚体数量。在分化 12 天时,老肌管的发生率、大小和完整四聚体数量与分化 9 天时的年轻肌管相当。总之,这些结果表明,在老年肌管中,参与兴奋-收缩耦合机制的蛋白质(如DHPR)的合成过程在某种程度上减慢了,这也支持了之前关于老年肌管分化潜力下降的研究证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
An aged-related structural study of DHPR tetrads in peripheral couplings of human skeletal muscle.

Among the numerous changes that occur in skeletal muscle during aging, the reduced regeneration potential after an injury is largely due to the impaired ability of satellite cells to proliferate and differentiate. Herein, using the freeze-fracture electron microscopy technique, we analyzed both the incidence and size of dihydropyridine receptors (DHPRs) tetrads (4 particles) in cultured myotubes from a young subject (28 years) after 9 days of differentiation and from an old subject (71 years) after 9 and 12 days of differentiation. Compared to young myotubes, at 9 days of differentiation old myotubes exhibited: i) a lower incidence and a smaller size of DHPR clusters and ii) a lower number of complete tetrads. At 12 days of differentiation values of incidence, size and number of complete tetrads in old myotubes were instead comparable with those of young myotubes at 9 days of differentiation. Collectively, these results indicate that in aged myotubes the synthesis process of the proteins involved in the excitation-contraction coupling mechanism, such as the DHPR, is somehow slowed, supporting previous studies evidence of a decrease in the differentiation potential of myotubes from elderly individuals.

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来源期刊
European Journal of Translational Myology
European Journal of Translational Myology MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
3.30
自引率
27.30%
发文量
74
审稿时长
10 weeks
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