{"title":"从土壤中分离出耐受重金属和抗植物病原菌的植物生长促进细菌。","authors":"Soo Yeon Lee, Kyung-Suk Cho","doi":"10.4014/jmb.2407.07013","DOIUrl":null,"url":null,"abstract":"<p><p>In this study, by isolating multifunctional soil bacteria that can promote plant development, resist heavy metals, exhibit anti-phytopathogenic action against plant diseases, and produce extracellular enzymes, we hope to improve the effectiveness of phytoremediation techniques. To isolate multifunctional soil bacteria, we used soils with diverse characteristics as isolation sources. To look into the diversity and structural traits of the bacterial communities, We conducted amplicon sequencing of the 16S rRNA gene on five types of soils and predicted functional genes using Tax4Fun2. The isolated bacteria were evaluated for their multifunctional capabilities, including heavy metal tolerance, plant growth promotion, anti-phytopathogenic activity, and extracellular enzyme activity. The genes related to plant growth promotion and anti-phytopathogenic activity were most abundant in forest and paddy soils. <i>Burkholderia</i> sp. FZ3 and FZ5 demonstrated excellent heavy metal resistance (≤ 1 mM Cd and ≤ 10 mM Zn), <i>Pantoea</i> sp. FC24 exhibited the highest protease activity (24.90 μmol tyrosine·g-DCW-<sup>1</sup>·h<sup>-1</sup>), and <i>Enterobacter</i> sp. PC20 showed superior plant growth promotion, especially in siderophore production. The multifunctional bacteria isolated using traditional methods included three strains (FC24, FZ3, and FZ5) from the forest and one strain (PC20) from paddy field soil. These results indicate that, for the isolation of beneficial soil microorganisms, utilizing target gene information obtained from isolation sources and subsequently exploring target microorganisms is a valuable strategy.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isolation of Heavy Metal-Tolerant and Anti-Phytopathogenic Plant Growth-Promoting Bacteria from Soils.\",\"authors\":\"Soo Yeon Lee, Kyung-Suk Cho\",\"doi\":\"10.4014/jmb.2407.07013\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In this study, by isolating multifunctional soil bacteria that can promote plant development, resist heavy metals, exhibit anti-phytopathogenic action against plant diseases, and produce extracellular enzymes, we hope to improve the effectiveness of phytoremediation techniques. To isolate multifunctional soil bacteria, we used soils with diverse characteristics as isolation sources. To look into the diversity and structural traits of the bacterial communities, We conducted amplicon sequencing of the 16S rRNA gene on five types of soils and predicted functional genes using Tax4Fun2. The isolated bacteria were evaluated for their multifunctional capabilities, including heavy metal tolerance, plant growth promotion, anti-phytopathogenic activity, and extracellular enzyme activity. The genes related to plant growth promotion and anti-phytopathogenic activity were most abundant in forest and paddy soils. <i>Burkholderia</i> sp. FZ3 and FZ5 demonstrated excellent heavy metal resistance (≤ 1 mM Cd and ≤ 10 mM Zn), <i>Pantoea</i> sp. FC24 exhibited the highest protease activity (24.90 μmol tyrosine·g-DCW-<sup>1</sup>·h<sup>-1</sup>), and <i>Enterobacter</i> sp. PC20 showed superior plant growth promotion, especially in siderophore production. The multifunctional bacteria isolated using traditional methods included three strains (FC24, FZ3, and FZ5) from the forest and one strain (PC20) from paddy field soil. These results indicate that, for the isolation of beneficial soil microorganisms, utilizing target gene information obtained from isolation sources and subsequently exploring target microorganisms is a valuable strategy.</p>\",\"PeriodicalId\":16481,\"journal\":{\"name\":\"Journal of microbiology and biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-10-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of microbiology and biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.4014/jmb.2407.07013\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of microbiology and biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.4014/jmb.2407.07013","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
在本研究中,我们希望通过分离能够促进植物生长、抗重金属、抗植物病害和产生胞外酶的多功能土壤细菌,来提高植物修复技术的效果。为了分离多功能土壤细菌,我们使用了具有不同特征的土壤作为分离源。为了研究细菌群落的多样性和结构特征,我们对五种土壤的 16S rRNA 基因进行了扩增测序,并使用 Tax4Fun2 预测了功能基因。对分离出的细菌进行了多功能能力评估,包括重金属耐受性、植物生长促进、抗植物病原菌活性和胞外酶活性。与促进植物生长和抗植物病原菌活性相关的基因在森林土壤和水稻田土壤中含量最高。伯克霍尔德氏菌 FZ3 和 FZ5 表现出优异的抗重金属能力(≤ 1 mM Cd 和 ≤ 10 mM Zn),泛变形菌 FC24 表现出最高的蛋白酶活性(24.90 μmol 酪氨酸-g-DCW-1-h-1),肠杆菌 PC20 表现出优异的植物生长促进能力,尤其是在苷元生产方面。用传统方法分离的多功能细菌包括来自森林的三株菌株(FC24、FZ3 和 FZ5)和来自水田土壤的一株菌株(PC20)。这些结果表明,在分离有益的土壤微生物时,利用从分离源获得的目标基因信息并随后探索目标微生物是一种有价值的策略。
Isolation of Heavy Metal-Tolerant and Anti-Phytopathogenic Plant Growth-Promoting Bacteria from Soils.
In this study, by isolating multifunctional soil bacteria that can promote plant development, resist heavy metals, exhibit anti-phytopathogenic action against plant diseases, and produce extracellular enzymes, we hope to improve the effectiveness of phytoremediation techniques. To isolate multifunctional soil bacteria, we used soils with diverse characteristics as isolation sources. To look into the diversity and structural traits of the bacterial communities, We conducted amplicon sequencing of the 16S rRNA gene on five types of soils and predicted functional genes using Tax4Fun2. The isolated bacteria were evaluated for their multifunctional capabilities, including heavy metal tolerance, plant growth promotion, anti-phytopathogenic activity, and extracellular enzyme activity. The genes related to plant growth promotion and anti-phytopathogenic activity were most abundant in forest and paddy soils. Burkholderia sp. FZ3 and FZ5 demonstrated excellent heavy metal resistance (≤ 1 mM Cd and ≤ 10 mM Zn), Pantoea sp. FC24 exhibited the highest protease activity (24.90 μmol tyrosine·g-DCW-1·h-1), and Enterobacter sp. PC20 showed superior plant growth promotion, especially in siderophore production. The multifunctional bacteria isolated using traditional methods included three strains (FC24, FZ3, and FZ5) from the forest and one strain (PC20) from paddy field soil. These results indicate that, for the isolation of beneficial soil microorganisms, utilizing target gene information obtained from isolation sources and subsequently exploring target microorganisms is a valuable strategy.
期刊介绍:
The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.