冷水浸泡通过调节泛素蛋白酶体相关蛋白，调节大鼠骨骼肌在偏心运动后的 NLRP3 炎症小体通路。

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine Pub Date : 2024-10-29 DOI:10.1016/j.cyto.2024.156793

Farzaneh Abolfathi , Rouhollah Ranjbar , Mohammad Reza Tabandeh , Abdolhamid Habibi

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引用次数: 0

摘要

背景：偏心运动（ECC）可诱发骨骼肌组织中与 NLRP3 相关的炎症。有限的现有数据显示，ECC后冷水浸泡（CWI）可抑制骨骼肌炎症。本研究旨在探讨 ECC 后冷水浸泡对大鼠骨骼肌中 NLRP3 炎症小体通路和泛素蛋白酶体相关蛋白（UPPs）表达的影响：将 25 只雄性 Wistar 大鼠随机分为对照组、ECC 组、ECC + CWI 组、ECC + NWI 组（正常水浸泡）和 ECC + AR 组（积极恢复）。偏心运动包括在速度为 16 米/分钟、坡度为 -16° 的跑步机上进行 90 分钟的下坡跑步。偏心运动后，NWI 组和 CWI 组的动物分别浸泡在 25°C 和 10°C 的水中。最后，分离比目鱼肌，并用Western印迹法评估NLRP3、caspase-1、FBXL2、TRIM31和PARKIN的表达。用 ELISA 法检测组织中 IL-1β 和 IL-18 的水平：结果：与对照组相比，ECC明显增加了NLRP3、caspase-1的表达以及组织中IL-1β和IL-18的水平。ECC后，FBXL2和PARKIN被下调，而TRIM31被上调（P 结论：CWI增加了NLRP3、Caspase-1和IL-18的表达：我们的研究结果表明，CWI 增加了 NLRP3 相关 UPPs 的表达，同时抑制了 ECC 后大鼠比目鱼肌中 NLRP3 的表达。因此，CWI 对减轻骨骼肌炎症的有益作用可能是由于 UPPs 表达的改变。

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Cold water immersion regulates NLRP3 inflammasome pathway in the rat skeletal muscle after eccentric exercise by regulating the ubiquitin proteasome related proteins

Background

Eccentric exercise (ECC) can induce NLRP3-related inflammation in skeletal muscle tissue. Limited available data have shown that Cold water immersion (CWI) after ECC can suppress skeletal muscle inflammation. This study aims to investigate the effect of CWI after ECC on the NLRP3 inflammasome pathway, and the expression of ubiquitin–proteasome-related proteins (UPPs) in the skeletal muscle of rats.

Methods

Twenty-five male Wistar rats were randomly divided into control, ECC, ECC + CWI, ECC + NWI (normal water immersion), and ECC + AR (active recovery) groups. The Eccentric exercise consisted of 90 min of downhill running on a treadmill with a speed of 16 m/min and −16° incline. Animals in the NWI and CWI groups were immersed in water at 25 °C and 10 °C after ECC. Eventually, The soleus muscle was isolated and the expression of NLRP3, caspase-1, FBXL2, TRIM31, and PARKIN was evaluated by western blot. Tissue levels of IL-1β and IL-18 were measured by ELISA assay.

Results

ECC significantly increased the expression of NLRP3, caspase-1, and the tissue levels of IL-1β and IL-18 compared to the control group. After ECC, FBXL2, and PARKIN were downregulated, whereas TRIM31 was up-regulated (P < 0.05). CWI after ECC suppressed the NLRP3 inflammasome components and increased the protein levels of FBXL2 and TRIM31 at higher levels than other recovery methods (P < 0.05). CWI and AR had the same increase in PARKIN expression and the same decrease in CK level compared to the ECC group (P < 0.05).

Conclusion

Our results indicated that CWI increased the expression of NLRP3-related UPPs in concomitant with suppression of NLRP3 in the soleus muscle of rats after ECC. As a result the beneficial effects of CWI on the attenuation of skeletal muscle inflammation may contribute to an alteration of UPPs expression.

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来源期刊

Cytokine 医学-免疫学

CiteScore

7.60

自引率

2.60%

发文量

262

审稿时长

48 days

期刊介绍： The journal Cytokine has an open access mirror journal Cytokine: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. * Devoted exclusively to the study of the molecular biology, genetics, biochemistry, immunology, genome-wide association studies, pathobiology, diagnostic and clinical applications of all known interleukins, hematopoietic factors, growth factors, cytotoxins, interferons, new cytokines, and chemokines, Cytokine provides comprehensive coverage of cytokines and their mechanisms of actions, 12 times a year by publishing original high quality refereed scientific papers from prominent investigators in both the academic and industrial sectors. We will publish 3 major types of manuscripts: 1) Original manuscripts describing research results. 2) Basic and clinical reviews describing cytokine actions and regulation. 3) Short commentaries/perspectives on recently published aspects of cytokines, pathogenesis and clinical results.