BCL11b 与涉及 RNA 处理和发育疾病的 RNA 和蛋白质相互作用。

IF 2.6 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Haitham Sobhy , Marco De Rovere , Amina Ait-Ammar , Muhammad Kashif , Clementine Wallet , Fadoua Daouad , Thomas Loustau , Carine Van Lint , Christian Schwartz , Olivier Rohr
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引用次数: 0

摘要

BCL11b 是一种转录调节因子和肿瘤抑制因子,参与淋巴瘤的发生、中枢神经系统(CNS)和免疫系统的发育。BCL11b 有利于艾滋病毒潜伏期的持续,并有助于控制多种生物体和细胞模型中的细胞周期、分化和凋亡。虽然BCL11b招募非编码RNA 7SK和表观遗传酶来调控基因表达,但BCL11b相关的核糖核蛋白复合物却不为人知。通过 CLIP-seq 和定量 LC-MS/MS 质谱方法以及系统生物学验证,我们发现 BCL11b 与 RNA 剪接和非有义介导衰变蛋白(包括 FUS、SMN1、UPF1 和 Drosha)相互作用,这可能有助于从非编码 RNA 异构体(保留内含子或无义介导 RNA)中选择蛋白编码 RNA 异构体。有趣的是,BCL11b 与相同基因(FUS、ESWR1、CHD 和 Tubulin)编码的 RNA 转录本和蛋白质结合。我们的研究强调,BCL11b 的靶标是 RNA 处理和剪接蛋白,以及与细胞周期、发育、神经退行性病变和癌症通路有关的 RNA。这些发现将有助于未来对发育障碍的机理认识。重要性:BCL11b与蛋白质和RNA的相互作用组揭示了BLC11b与参与基因表达调控的特定核蛋白复合物的关联。BCL11b与RNA加工和剪接蛋白相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
BCL11b interacts with RNA and proteins involved in RNA processing and developmental diseases
BCL11b is a transcription regulator and a tumor suppressor involved in lymphomagenesis, central nervous system (CNS) and immune system developments. BCL11b favors persistence of HIV latency and contributes to control cell cycle, differentiation and apoptosis in multiple organisms and cell models. Although BCL11b recruits the non-coding RNA 7SK and epigenetic enzymes to regulate gene expression, BCL11b-associated ribonucleoprotein complexes are unknown. Thanks to CLIP-seq and quantitative LC-MS/MS mass spectrometry approaches complemented with systems biology validations, we show that BCL11b interacts with RNA splicing and non-sense-mediated decay proteins, including FUS, SMN1, UPF1 and Drosha, which may contribute in isoform selection of protein-coding RNA isoforms from noncoding-RNAs isoforms (retained introns or nonsense mediated RNA). Interestingly, BCL11b binds to RNA transcripts and proteins encoded by the same genes (FUS, ESWR1, CHD and Tubulin). Our study highlights that BCL11b targets RNA processing and splicing proteins, and RNAs that implicate cell cycle, development, neurodegenerative, and cancer pathways. These findings will help future mechanistic understanding of developmental disorders.

Importance

BCL11b-protein and RNA interactomes reveal BLC11b association with specific nucleoprotein complexes involved in the regulation of genes expression.
BCL11b interacts with RNA processing and splicing proteins.
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来源期刊
CiteScore
9.20
自引率
2.10%
发文量
63
审稿时长
44 days
期刊介绍: BBA Gene Regulatory Mechanisms includes reports that describe novel insights into mechanisms of transcriptional, post-transcriptional and translational gene regulation. Special emphasis is placed on papers that identify epigenetic mechanisms of gene regulation, including chromatin, modification, and remodeling. This section also encompasses mechanistic studies of regulatory proteins and protein complexes; regulatory or mechanistic aspects of RNA processing; regulation of expression by small RNAs; genomic analysis of gene expression patterns; and modeling of gene regulatory pathways. Papers describing gene promoters, enhancers, silencers or other regulatory DNA regions must incorporate significant functions studies.
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