Hsa_circ_0008667 通过抑制 miR-9-5p 促进胃癌的进展并改善其预后。

IF 1.1 4区 医学 Q4 GASTROENTEROLOGY & HEPATOLOGY
Wei Ding, Zhengliang Li, Xiaojing Liu, Jundong Wang, Jiaxi Wang, Guoxiang Jiang, Haizhou Yu, Tuya Wang
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引用次数: 0

摘要

研究背景和目的:胃癌(GC)是最常见的消化道肿瘤之一,具有侵袭性发展和预后不良的特点。环状 RNA(circRNA)已被用作包括胃癌在内的许多癌症的预后生物标志物和治疗靶点。Hsa_circ_0008667 在 GC 中有差异表达,但其功能和临床意义仍未得到阐明。因此,本研究旨在探讨 Hsa_circ_0008667 在 GC 中的作用和意义,以及其作为 GC 生物标志物和治疗靶点的潜力:通过反转录实时定量PCR技术分析hsa_circ_0008667在GC组织和细胞中的表达情况,并通过统计学分析评估其临床意义。通过细胞计数试剂盒-8和Transwell试验检测沉默hsa_circ_0008667对GC细胞生长和转移的影响。此外,还进行了相关性分析,以评估 hsa_circ_0008667 与 miR-9-5p 之间的关系,并通过荧光素酶报告实验进一步验证了这种关系:结果:Hsa_circ_0008667显著上调,并与淋巴结转移和肿瘤-结节-转移分期密切相关,可预测GC患者的不良预后。沉默 Hsa_circ_0008667 可抑制 GC 细胞的增殖、迁移和侵袭。此外,hsa_circ_0008667还能负向调节miR-9-5p的表达。MiR-9-5p的下调增强了GC的恶性程度,并逆转了hsa_circ_0008667敲除介导的GC抑制作用:结论:本研究结果表明,hsa_circ_0008667可通过调控miR-9-5p成为GC的预后生物标志物和肿瘤促进因子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hsa_circ_0008667 promotes progression and improves the prognosis of gastric cancer by inhibiting miR-9-5p.

Background and study aim: Gastric cancer (GC) is one of the most common gastrointestinal tumors characterized by aggressive development and poor prognosis. Circular RNAs (circRNAs) have been used as prognostic biomarkers and therapeutic targets in many cancers, including GC. Hsa_circ_0008667 is differentially expressed in GC; however, its function and clinical significance remained unelucidated. Therefore, this study aimed to investigate the role and significance of hsa_circ_0008667 in GC and its potential as a biomarker and therapeutic target of GC.

Patients and methods: Through quantitative reverse-transcription real-time PCR, hsa_circ_0008667 expression in GC tissues and cells were analyzed, followed by statistical analyses to assess the clinical significance. Cell Counting Kit-8 and Transwell assays were performed to examine the effects of hsa_circ_0008667 silencing on GC cell growth and metastasis. Additionally, correlation analysis was performed to assess the relationship between hsa_circ_0008667 and miR-9-5p, which was further validated through luciferase reporter assay.

Results: Hsa_circ_0008667 was considerably upregulated and tightly correlated with lymph node metastasis and the tumor-node-metastasis stage, which was predictive of poor prognosis in patients with GC. Hsa_circ_0008667 silencing suppressed GC cell proliferation, migration, and invasion. Furthermore, hsa_circ_0008667 negatively regulated miR-9-5p expression. MiR-9-5p downregulation enhanced GC malignancy and reversed hsa_circ_0008667 knockdown-mediated GC suppression.

Conclusion: The findings of this study suggest hsa_circ_0008667 to be a prognostic biomarker and tumor promoter of GC via miR-9-5p modulation.

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来源期刊
Arab Journal of Gastroenterology
Arab Journal of Gastroenterology Medicine-Gastroenterology
CiteScore
2.70
自引率
0.00%
发文量
52
期刊介绍: Arab Journal of Gastroenterology (AJG) publishes different studies related to the digestive system. It aims to be the foremost scientific peer reviewed journal encompassing diverse studies related to the digestive system and its disorders, and serving the Pan-Arab and wider community working on gastrointestinal disorders.
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