检测抗 HPV16 L1 特异性 IgG1、IgG3、IgA 和 IgM 抗体的酶联免疫吸附测定的重现性评估

IF 5.2 3区 医学 Q1 IMMUNOLOGY
Vaccines Pub Date : 2024-09-27 DOI:10.3390/vaccines12101108
Ken Matsui, Heidi Anne Hempel, Gloriana Shelton, Rebecca Ocampo, Troy J Kemp, Yuanji Pan, Ligia A Pinto
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引用次数: 0

摘要

背景/目的:酶联免疫吸附试验(ELISA)已被用于测量抗人类乳头瘤病毒(HPV)免疫球蛋白 IgG。本研究的目的是评估 ELISAs 测量不同 HPV 免疫球蛋白异型(IgG1、2、3 和 4、IgA 和 IgM)抗 HPV16 的重现性:从哥斯达黎加 HPV 疫苗试验 (CVT) 参与者身上采集的 72 份血清样本用于重现性评估,这些样本接种了二价 HPV 疫苗 (2vHPV)。IgG2和IgG4水平太低,无法检测。对 IgG1、IgG3、IgA 和 IgM 的水平进行了测量,并用这些数据计算了类内相关系数 (ICC) 和变异系数 (CV):评估了技术人员之间(12.8%-22.7%)和不同天之间(6.2%-30.6%)的变异系数。总体变异系数为 7.7-31.1%。IgM ELISA 的 CV 值(15.8-31.1%)高于 IgG1、IgG3 和 IgA(6.2-22.7%)。所有 ICC 值均大于 98.7%。所有样本中都检测到了 IgG3,而 IgG1 和 IgA 的检出率大于 86.3%,IgM 的检出率为 62.1%。不同抗体之间的皮尔逊相关性分析均显示出显著的相关性(p ≤ 0.001),但 IgG 或 IgA 与 IgM 的比较除外(p = 0.29-0.53):我们的数据表明,这些酶联免疫吸附试验具有可重复性,能在接种过2vHPV疫苗的参与者中检测到一定浓度范围内的HPV16 L1同型抗体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Reproducibility Assessment of Enzyme-Linked Immunosorbent Assays to Detect Anti-HPV16 L1-Specific IgG1, IgG3, IgA, and IgM Antibodies.

Background/objectives: Enzyme-linked immunosorbent assays (ELISAs) have been used to measure anti-human-papillomavirus (HPV) immunoglobulin IgG. The goal of this study was to evaluate the reproducibility of ELISAs measuring different HPV immunoglobulin isotypes, IgG1, 2, 3, and 4, IgA, and IgM, against HPV16.

Methods: Seventy-two serum samples collected from participants in the Costa Rica HPV Vaccine Trial (CVT) and immunized with bivalent HPV vaccine (2vHPV) were used for reproducibility assessment. IgG2 and IgG4 levels were too low to be detected. Levels of IgG1, IgG3, IgA, and IgM were measured, and the data were used to calculate intraclass correlation coefficients (ICCs) and coefficients of variation (CVs).

Results: CVs were assessed between technicians (12.8-22.7%) and across days (6.2-30.6%). The overall CVs ranged from 7.7-31.1%. IgM ELISA showed higher CVs (15.8-31.1%) than IgG1, IgG3, and IgA (6.2-22.7%). All ICC values were >98.7%. IgG3 was detected in all samples, while IgG1 and IgA had >86.3% detectability and IgM had 62.1% detectability. Pearson correlational analyses between different antibodies all showed significant correlations (p ≤ 0.001), except when comparing IgGs or IgA to IgM (p = 0.29-0.53).

Conclusions: Our data showed that these ELISAs are reproducible and detect isotype antibodies to HPV16 L1 across a range of concentrations in 2vHPV-vaccinated participants.

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来源期刊
Vaccines
Vaccines Pharmacology, Toxicology and Pharmaceutics-Pharmacology
CiteScore
8.90
自引率
16.70%
发文量
1853
审稿时长
18.06 days
期刊介绍: Vaccines (ISSN 2076-393X) is an international, peer-reviewed open access journal focused on laboratory and clinical vaccine research, utilization and immunization. Vaccines publishes high quality reviews, regular research papers, communications and case reports.
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