当代荧光相关光谱法在各种溶液研究中的应用综述。

IF 7.2 2区生物学 Q1 BIOPHYSICS

Quarterly Reviews of Biophysics Pub Date : 2024-10-28 DOI:10.1017/S003358352400012X

Snežana M Jovičić

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引用次数: 0

摘要

荧光相关光谱法（FCS）是一种著名的非侵入式方法，用于量化分子机制和动力学的物理参数。它结合了最大灵敏度和统计置信度，通过在定义明确的体积元素中进行时间平均波动分析，分析荧光分子的速度、大小和数量以及与周围分子的相互作用。本研究的范围较窄，主要介绍扩散的基本原理和 FCS 方法在变量大小和标准化调整方面的一般原理。在这篇综述中，我们给出了理论介绍、实验应用实例以及溶液系统中的用具和未来展望。

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Review of contemporary fluorescence correlation spectroscopy method in diverse solution studies.

Fluorescence correlation spectroscopy (FCS) is a well-known and established non-invasive method for quantification of physical parameters that preside over molecular mechanisms and dynamics. It combines maximum sensitivity and statistical confidence for the analysis of speed, size, and number of fluorescent molecules and interactions with surrounding molecules by time-averaging fluctuation analysis in a well-defined volume element. The narrow compass of this study is to acquaint the basic principle of diffusion and the FCS method in general regarding variable magnitudes and standardization adjustment. In this review, we give a theoretical introduction, examples of experimental applications, and utensils in solution systems with future perspectives.

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来源期刊

Quarterly Reviews of Biophysics 生物-生物物理

CiteScore

12.90

自引率

1.60%

发文量

期刊介绍： Quarterly Reviews of Biophysics covers the field of experimental and computational biophysics. Experimental biophysics span across different physics-based measurements such as optical microscopy, super-resolution imaging, electron microscopy, X-ray and neutron diffraction, spectroscopy, calorimetry, thermodynamics and their integrated uses. Computational biophysics includes theory, simulations, bioinformatics and system analysis. These biophysical methodologies are used to discover the structure, function and physiology of biological systems in varying complexities from cells, organelles, membranes, protein-nucleic acid complexes, molecular machines to molecules. The majority of reviews published are invited from authors who have made significant contributions to the field, who give critical, readable and sometimes controversial accounts of recent progress and problems in their specialty. The journal has long-standing, worldwide reputation, demonstrated by its high ranking in the ISI Science Citation Index, as a forum for general and specialized communication between biophysicists working in different areas. Thematic issues are occasionally published.