Paola Quifer-Rada, Laia Aguilar-Camprubí, Sara Samino, Nuria Amigó, Oria Soler, Alba Padró-Arocas
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To assess the association between the significant metabolites and lipids and the development of acute and subacute mastitis, multi-adjusted logistic regression models were developed. <b>Results:</b> The NMR-based metabolomics approach was able to identify and quantify a total of 40 metabolites in breast milk samples. After adjusting for confounding variables, acute mastitis was significantly associated with acetate (OR 3.9 IC 1.4-10.8), total cholesterol (OR 14 CI 3.2-62), esterified cholesterol (OR 3.3 CI 1.9-5.8), and sphingomyelin (OR 2.6 CI 1.2-5.8). The other metabolites presented weak association (OR < 2.5). Subacute mastitis was significantly associated with glutamine, lysophosphatidylcholine, phosphatidylcholine, plasmalogen, and total polyunsaturated fatty acids, but only cholesterol showed a strong association (OR > 2.5) with an OR of 2.6 (IC 1.1-6.6). <b>Conclusions</b>: Metabolic alteration in breast milk occurs during a process of both acute and subacute mastitis. 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引用次数: 0
摘要
背景和目的:乳腺炎是母乳喂养期间的主要并发症之一,也是导致停止母乳喂养的原因之一。然而,乳腺炎的发病机制和诊断非常复杂,尚未得到很好的界定。我们旨在确定急性和亚急性乳腺炎期间母乳中的代谢和脂质变化,以检测乳腺炎的潜在生物标志物。研究方法我们进行了一项试验性病例对照研究,其中包括 14 名患有急性乳腺炎的哺乳期妇女、32 名有亚急性乳腺炎症状的妇女和 19 名没有任何乳腺炎症状的妇女(对照组)。研究人员收集了牛奶样本,并通过质子核磁共振(H-NMR)进行代谢组学分析。为了评估重要代谢物和脂质与急性和亚急性乳腺炎发病之间的关系,建立了多重调整逻辑回归模型。结果基于核磁共振的代谢组学方法能够鉴定和量化母乳样本中的 40 种代谢物。在对混杂变量进行调整后,急性乳腺炎与醋酸盐(OR 3.9 IC 1.4-10.8)、总胆固醇(OR 14 CI 3.2-62)、酯化胆固醇(OR 3.3 CI 1.9-5.8)和鞘磷脂(OR 2.6 CI 1.2-5.8)显著相关。其他代谢物的相关性较弱(OR < 2.5)。亚急性乳腺炎与谷氨酰胺、溶血磷脂酰胆碱、磷脂酰胆碱、质氨酰胆碱和多不饱和脂肪酸总量有明显相关性,但只有胆固醇显示出较强的相关性(OR > 2.5),OR 为 2.6(IC 1.1-6.6)。结论在急性和亚急性乳腺炎的过程中,母乳中会发生代谢变化。在急性和亚急性乳腺炎中,乙酸盐、酯化胆固醇、溶血胆碱和多不饱和脂肪酸都会增加。然而,根据多重调整回归逻辑模型,急性和亚急性乳腺炎的候选生物标志物是胆固醇、溶血磷脂酰胆碱、磷脂酰胆碱、质脲原和多不饱和脂肪酸。
Metabolomics Approach to Identify Biomarkers of Acute and Subacute Mastitis in Milk Samples: A Pilot Case-Control Study.
Background and aims: Mastitis is one of the main complications during breastfeeding and contributes to the cessation of breastfeeding. However, the etiopathogenesis and diagnosis of mastitis are complex and not yet well defined. We aimed to identify metabolic and lipidic changes in human milk during acute and subacute mastitis in order to detect potential biomarkers of mastitis. Methods: We conducted a pilot case-control study including 14 breastfeeding women with acute mastitis, 32 with subacute mastitis symptoms, and 19 without any mastitis symptoms (control). Milk samples were collected and analyzed by proton nuclear magnetic resonance (H-NMR) for metabolomics analysis. To assess the association between the significant metabolites and lipids and the development of acute and subacute mastitis, multi-adjusted logistic regression models were developed. Results: The NMR-based metabolomics approach was able to identify and quantify a total of 40 metabolites in breast milk samples. After adjusting for confounding variables, acute mastitis was significantly associated with acetate (OR 3.9 IC 1.4-10.8), total cholesterol (OR 14 CI 3.2-62), esterified cholesterol (OR 3.3 CI 1.9-5.8), and sphingomyelin (OR 2.6 CI 1.2-5.8). The other metabolites presented weak association (OR < 2.5). Subacute mastitis was significantly associated with glutamine, lysophosphatidylcholine, phosphatidylcholine, plasmalogen, and total polyunsaturated fatty acids, but only cholesterol showed a strong association (OR > 2.5) with an OR of 2.6 (IC 1.1-6.6). Conclusions: Metabolic alteration in breast milk occurs during a process of both acute and subacute mastitis. Acetate, esterified cholesterol, lysophostidylcholine, and polyunsaturated fatty acids increased in both acute and subacute mastitis. However, according to the multi-adjusted regression logistic models, the candidate biomarkers for acute and subacute mastitis are cholesterol, lysophosphatidylcoholine, phosphatidylcholine, plasmalogen, and polyunsaturated fatty acids.
MetabolitesBiochemistry, Genetics and Molecular Biology-Molecular Biology
CiteScore
5.70
自引率
7.30%
发文量
1070
审稿时长
17.17 days
期刊介绍:
Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.