基于包膜糖蛋白的多重免疫测定在一组埃博拉病毒病幸存者中检测埃博拉病毒抗体的性能。

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of virological methods Pub Date : 2024-10-24 DOI:10.1016/j.jviromet.2024.115057

McKenna D. Roe , Grace Hood , Spencer L. Sterling , Lianying Yan , Joseph Akoi Boré , Tom Tipton , Craig Thompson , Miles W. Carroll , Eric D. Laing

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引用次数: 0

摘要

对动物和人类宿主进行血清学监测是一种具有成本效益的正粘病毒检测策略，但在准确估计血清流行率、潜在的无症状疾病表现和抗原交叉反应等方面面临挑战。在此，我们介绍了一种基于包膜糖蛋白（GP）的多重微球免疫测定方法，该方法由九种丝状病毒 GP 抗原组成，用于检测几内亚埃博拉病毒病（EVD）幸存者和 2013 - 2016 年西非埃博拉病毒病爆发接触者队列中的抗埃博拉病毒（EBOV）抗体。我们检测了以重组三聚体外显子形式表达的 GP 检测抗 EBOV 抗体的灵敏度和特异性，结果显示检测灵敏度为 95.96%，特异性为 98.61%。此外，多重检测与全病毒酶联免疫吸附和病毒中和检测之间的一致性显示出很强的相关性。结果表明，这种丝状病毒多重检验是高通量血清监测的灵敏工具。

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Performance of an envelope glycoprotein-based multiplex immunoassay for Ebola virus antibody detection in a cohort of Ebola virus disease survivors

Serological surveillance in animal and human hosts can be a cost-effective strategy for orthoebolavirus detection, but is challenged by accurate estimates of seroprevalence, potential pauci-symptomatic disease presentation, and antigenic cross-reactivity. Here, we describe the use of an envelope glycoprotein (GP)-based multiplex microsphere immunoassay, consisting of nine filovirus GP antigens for the detection of anti-Ebola virus (EBOV) antibodies in a well-characterized cohort of Guinean Ebola virus disease (EVD) survivors and contacts from the 2013 – 2016 West African EVD outbreak. We examined sensitivity and specificity for the detection of anti-EBOV antibodies by GP expressed as recombinant trimeric ectodomains, yielding an assay performance of 95.96 % sensitivity and 98.61 % specificity. Additionally, agreement between the multiplex test and a whole virus ELISA and virus neutralization test showed strong correlations. The results demonstrate that this filovirus multiplex test is a sensitive tool for high-throughput serosurveillance

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来源期刊

Journal of virological methods 医学-病毒学

CiteScore

5.80

自引率

0.00%

发文量

209

审稿时长

41 days

期刊介绍： The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.