葶苈子提取物的降血糖特性--关于α-葡萄糖苷酶和α-淀粉酶抑制作用及代谢谱测定的体外研究

IF 4.2 2区 生物学 Q2 MICROBIOLOGY
Valeria Ferraro, Anna Spagnoletta, Natalie Paola Rotondo, René Massimiliano Marsano, Daniela Valeria Miniero, Gaetano Balenzano, Annalisa De Palma, Alessandro Colletti, Maria Letizia Gargano, Giovanni Lentini, Maria Maddalena Cavalluzzi
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引用次数: 0

摘要

2 型糖尿病影响着越来越多的世界人口,而通过饮食管理(包括食用促进健康的食品或其衍生补充剂)来控制病情是一种常见的策略。有几种蘑菇被证明具有抗糖尿病的特性,这是因为它们能够改善胰岛素的敏感性和分泌,或抑制碳水化合物水解酶α-淀粉酶和α-葡萄糖苷酶。本研究旨在首次研究食用菌 Leccinum scabrum (Bull.) Gray 的降血糖特性。采用不同极性的绿色溶剂,通过微波辅助萃取(MAE)技术制备了蘑菇提取物。对所有提取物对α-葡萄糖苷酶和α-淀粉酶的抑制活性进行了评估,发现乙酸提取物的活性最高,其对α-葡萄糖苷酶的 IC50 值(分别为 0.42 ± 0.02 和 25.4 ± 0.6 µg/mL)比参考化合物 1-deoxynojirimycin (DNJ) 低约 60 倍。根据有关α-葡萄糖苷酶和α-淀粉酶抑制作用的文献数据,预计该物质对胰腺α-淀粉酶的抑制活性较弱。对活性最强的提取物(EtOAc)进行的果蝇体内初步试验证实了体外观察到的降血糖活性。最后,通过 GC-MS 和 HRMS 分析确定了 EtOAc 提取物的代谢概况。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hypoglycemic Properties of Leccinum scabrum Extracts-An In Vitro Study on α-Glucosidase and α-Amylase Inhibition and Metabolic Profile Determination.

Type-2 diabetes affects an increasing percentage of the world's population and its control through dietary management, involving the consumption of health-promoting foods or their derived supplements, is a common strategy. Several mushroom species have been demonstrated to be endowed with antidiabetic properties, resulting from their ability in improving insulin sensitivity and production, or inhibiting the carbohydrate-hydrolyzing enzymes α-amylase and α-glucosidase. This study aimed to investigate for the first time the hypoglycemic properties of the edible mushroom Leccinum scabrum (Bull.) Gray. Mushroom extracts were prepared through the microwave-assisted extraction (MAE) technique using green solvents with different polarity degrees. The inhibition activity of all the obtained extracts on both α-glucosidase and α-amylase was evaluated and the highest activity was observed for the EtOAc extract which showed an IC50 value about 60-fold lower than the reference compound 1-deoxynojirimycin (DNJ) on α-glucosidase (0.42 ± 0.02 and 25.4 ± 0.6 µg/mL, respectively). As expected on the basis of the literature data concerning both α-glucosidase and α-amylase inhibition, a milder inhibition activity on pancreatic α-amylase was observed. Preliminary in vivo tests on Drosophila melanogaster carried out on the most active obtained extract (EtOAc) confirmed the in vitro observed hypoglycemic activity. Finally, the EtOAc extract metabolic profile was determined through GC-MS and HRMS analyses.

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来源期刊
Journal of Fungi
Journal of Fungi Medicine-Microbiology (medical)
CiteScore
6.70
自引率
14.90%
发文量
1151
审稿时长
11 weeks
期刊介绍: Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.
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