Bactrocera tryoni 和 Ceratitis capitata 的不同热应激反应

IF 2.7 2区 农林科学 Q1 ENTOMOLOGY
Insects Pub Date : 2024-09-30 DOI:10.3390/insects15100759
Kay Anantanawat, Alexie Papanicolaou, Kelly Hill, Yalin Liao, Wei Xu
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引用次数: 0

摘要

入侵的表皮蝇类果蝇是全世界最具破坏性的农业和园艺害虫之一。通常采用热处理作为收获后灭杀水果或蔬菜中果蝇的方法。这些害虫物种对热处理表现出不同的耐受性,表明不同物种受热影响的分子途径可能不同。本研究以昆士兰果蝇(Qfly)--Bactrocera tryoni--为模型,通过热生物测定研究其对热应力的分子反应。研究人员提取了热处理前后果蝇的 RNA 样本并进行了测序,以确定表达发生显著变化的基因。在类似的热处理条件下,将这些发现与另一种严重的表皮蝇类果蝇--地中海果蝇(Medfly)--Ceratitis capitata进行了比较。分析结果显示,只有三个共同基因:热休克蛋白 70(HSP70)、HSP68 和 14-3-3 zeta 蛋白。然而,尽管存在这些共同基因,Qfly 和 Medfly 的表达模式却有所不同。这表明这些基因可能在每个物种的热反应中发挥不同的作用,并可能受到不同的调控。这项研究首次证明了 Qfly 和 Medfly 对热的不同分子反应,这可能与它们不同的起源、栖息地和遗传背景有关。这些发现提供了在分子水平上对Tephritid果蝇对热的反应的新见解,这可能有助于改进未来控制这些害虫的采后策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Divergent Heat Stress Responses in Bactrocera tryoni and Ceratitis capitata.

Invasive Tephritid fruit flies rank among the most destructive agricultural and horticultural pests worldwide. Heat treatment is commonly employed as a post-harvest method to exterminate fruit flies in fruits or vegetables. These pest species exhibit distinct tolerance to heat treatments, suggesting that the molecular pathways affected by heat may differ among species. In this study, the Queensland fruit fly (Qfly), Bactrocera tryoni, was utilised as a model investigate its molecular response to heat stress through heat bioassays. RNA samples from flies before and after heat treatment were extracted and sequenced to identify genes with significant changes in expression. These findings were compared to another serious Tephritid fruit fly species, the Mediterranean fruit fly (Medfly), Ceratitis capitata, under similar heat treatment conditions. The analysis reveals only three common genes: heat shock protein 70 (HSP70), HSP68, and 14-3-3 zeta protein. However, despite these shared genes, their expression patterns differ between Qfly and Medfly. This suggests that these genes might play different roles in the heat responses of each species and could be regulated differently. This study presents the first evidence of differing molecular responses to heat between Qfly and Medfly, potentially linked to their varied origins, habitats, and genetic backgrounds. These findings offer new insights into Tephritid fruit fly responses to heat at the molecular level, which may help refine post-harvest strategies to control these pests in the future.

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来源期刊
Insects
Insects Agricultural and Biological Sciences-Insect Science
CiteScore
5.10
自引率
10.00%
发文量
1013
审稿时长
21.77 days
期刊介绍: Insects (ISSN 2075-4450) is an international, peer-reviewed open access journal of entomology published by MDPI online quarterly. It publishes reviews, research papers and communications related to the biology, physiology and the behavior of insects and arthropods. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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