{"title":"对长江口黄颡鱼和鲤鱼肠道微生物区系的认识","authors":"Jiahao Wang, Guangpeng Feng, Zhiqiang Han, Tao Zhang, Jinhui Chen, Jianhui Wu","doi":"10.3389/fcimb.2024.1420928","DOIUrl":null,"url":null,"abstract":"<p><p>The gut microbiota plays a crucial role in food webs, carbon cycling, and related elements. <i>Exopalaemon annandalei</i> and <i>Exopalaemon carinicauda</i> are two important forage species in the Yangtze River estuary with extremely similar living habits and morphological characteristics. Exploring the microorganisms in the guts of these two shrimp species can help us understand the survival status of forage species and gut microbiota in the Yangtze River estuary. Therefore, this study analyzed the similarities and differences in the intestinal flora of <i>E. annandalei</i> and <i>E. carinicauda</i> through high-throughput sequencing of 16S rRNA gene amplicons. The results showed that the dominant bacteria in the intestinal flora of <i>E. annandalei</i> and <i>E. carinicauda</i> at the phylum level were Proteobacteria and Firmicutes, respectively. At the genus level, the intestinal flora had higher concentrations of <i>Psychrobacter</i>, <i>Bacillus</i>, <i>Pseudomonas</i>, <i>Acinetobacter</i>, and <i>Macrococcus</i>. In both shrimp species, the contents of <i>Acinetobacter</i> and <i>Macrococcus</i> were higher in spring than in winter. The most important potential functions of the intestinal microbiota were amino acid metabolism and purine metabolism. Additionally, the functions of metabolism and diseases in the intestinal microbiota of <i>E. annandalei</i> were greatly influenced by the season. Furthermore, the experimental results indicated that a lower ratio of <i>Firmicutes</i> to <i>Bacteroidetes</i> was associated with a larger body weight in shrimp. Overall, this study provides a theoretical reference for understanding the intestinal bacterial community of shrimp in estuaries and the healthy cultivation of <i>E. annandalei</i> and <i>E. carinicauda</i>.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"14 ","pages":"1420928"},"PeriodicalIF":4.6000,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11496289/pdf/","citationCount":"0","resultStr":"{\"title\":\"Insights into the intestinal microbiota of <i>Exopalaemon annandalei</i> and <i>Exopalaemon carinicauda</i> in the Yangtze River estuary.\",\"authors\":\"Jiahao Wang, Guangpeng Feng, Zhiqiang Han, Tao Zhang, Jinhui Chen, Jianhui Wu\",\"doi\":\"10.3389/fcimb.2024.1420928\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The gut microbiota plays a crucial role in food webs, carbon cycling, and related elements. <i>Exopalaemon annandalei</i> and <i>Exopalaemon carinicauda</i> are two important forage species in the Yangtze River estuary with extremely similar living habits and morphological characteristics. Exploring the microorganisms in the guts of these two shrimp species can help us understand the survival status of forage species and gut microbiota in the Yangtze River estuary. Therefore, this study analyzed the similarities and differences in the intestinal flora of <i>E. annandalei</i> and <i>E. carinicauda</i> through high-throughput sequencing of 16S rRNA gene amplicons. The results showed that the dominant bacteria in the intestinal flora of <i>E. annandalei</i> and <i>E. carinicauda</i> at the phylum level were Proteobacteria and Firmicutes, respectively. At the genus level, the intestinal flora had higher concentrations of <i>Psychrobacter</i>, <i>Bacillus</i>, <i>Pseudomonas</i>, <i>Acinetobacter</i>, and <i>Macrococcus</i>. In both shrimp species, the contents of <i>Acinetobacter</i> and <i>Macrococcus</i> were higher in spring than in winter. The most important potential functions of the intestinal microbiota were amino acid metabolism and purine metabolism. Additionally, the functions of metabolism and diseases in the intestinal microbiota of <i>E. annandalei</i> were greatly influenced by the season. Furthermore, the experimental results indicated that a lower ratio of <i>Firmicutes</i> to <i>Bacteroidetes</i> was associated with a larger body weight in shrimp. 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引用次数: 0
摘要
肠道微生物群在食物网、碳循环及相关要素中发挥着至关重要的作用。Exopalaemon annandalei和Exopalaemon carinicauda是长江口的两种重要饵料物种,它们的生活习性和形态特征极为相似。对这两种虾肠道微生物的研究有助于我们了解长江口饵料物种和肠道微生物群的生存状况。因此,本研究通过对 16S rRNA 基因扩增子进行高通量测序,分析了环带鰕虎鱼和胭脂鱼肠道菌群的异同。结果表明,在门一级,E. annandalei 和 E. carinicauda 肠道菌群的优势菌分别是变形菌和固着菌。在属的层面上,肠道菌群中的精神杆菌、芽孢杆菌、假单胞菌、醋杆菌和巨球菌含量较高。在两种对虾中,春季的醋氨杆菌和巨球菌含量均高于冬季。肠道微生物群最重要的潜在功能是氨基酸代谢和嘌呤代谢。此外,环带鳗肠道微生物群的代谢和疾病功能受季节影响很大。此外,实验结果表明,固着菌与类杆菌的比例越低,对虾的体重越大。总之,本研究为了解河口对虾肠道细菌群落以及健康养殖 E. annandalei 和 E. carinicauda 提供了理论参考。
Insights into the intestinal microbiota of Exopalaemon annandalei and Exopalaemon carinicauda in the Yangtze River estuary.
The gut microbiota plays a crucial role in food webs, carbon cycling, and related elements. Exopalaemon annandalei and Exopalaemon carinicauda are two important forage species in the Yangtze River estuary with extremely similar living habits and morphological characteristics. Exploring the microorganisms in the guts of these two shrimp species can help us understand the survival status of forage species and gut microbiota in the Yangtze River estuary. Therefore, this study analyzed the similarities and differences in the intestinal flora of E. annandalei and E. carinicauda through high-throughput sequencing of 16S rRNA gene amplicons. The results showed that the dominant bacteria in the intestinal flora of E. annandalei and E. carinicauda at the phylum level were Proteobacteria and Firmicutes, respectively. At the genus level, the intestinal flora had higher concentrations of Psychrobacter, Bacillus, Pseudomonas, Acinetobacter, and Macrococcus. In both shrimp species, the contents of Acinetobacter and Macrococcus were higher in spring than in winter. The most important potential functions of the intestinal microbiota were amino acid metabolism and purine metabolism. Additionally, the functions of metabolism and diseases in the intestinal microbiota of E. annandalei were greatly influenced by the season. Furthermore, the experimental results indicated that a lower ratio of Firmicutes to Bacteroidetes was associated with a larger body weight in shrimp. Overall, this study provides a theoretical reference for understanding the intestinal bacterial community of shrimp in estuaries and the healthy cultivation of E. annandalei and E. carinicauda.
期刊介绍:
Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.