循环长非编码 RNA SNHG1 在 2 型糖尿病中的临床意义及其与胰腺 β 细胞增殖的关系

IF 2.8 3区医学 Q3 ENDOCRINOLOGY & METABOLISM

BMC Endocrine Disorders Pub Date : 2024-10-25 DOI:10.1186/s12902-024-01755-6

Tianxiang Xu, Tuwang Shen, Song Yang, Yuan Li, Li Liu, Lili Du

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引用次数: 0

摘要

背景：探讨长非编码RNA（lncRNA）SNHG1/ miR-195轴与2型糖尿病（T2DM）和胰岛功能的关系：探讨长非编码RNA（lncRNA）SNHG1/ miR-195轴与2型糖尿病（T2DM）和胰岛功能的关系：方法：测定T2DM患者和健康人体内SNHG1和miR-195的表达。采用皮尔逊相关分析评估指标之间的相关性。INS-1 细胞用于进行细胞功能测定。使用 ELISA 检测 INS-1 细胞分泌胰岛素的情况。细胞计数试剂盒-8（CCK-8）和流式细胞仪用于检测细胞增殖和凋亡。荧光素酶报告试验用于验证 SNHG1 的靶标：结果：T2DM 患者血清中 SNHG1 表达增加，miR-195 水平降低。SNHG1和miR-195均可作为诊断T2DM的生物标志物。空腹血浆葡萄糖（FPG）和 HbA1c 与 SNHG1 呈正相关，而与 miR-195 呈负相关。SNHG1 通过与 miR-195 结合抑制 INS-1 细胞的胰岛素分泌、细胞增殖并促进其凋亡：结论：检测 SNHG1 和 miR-195 可预测 T2DM。结论：SNHG1和miR-195的检测可预测T2DM，SNHG1可抑制INS-1细胞的增殖和胰岛素分泌，但通过与miR-195结合可促进INS-1细胞的凋亡。

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Clinical significance of circulating long non-coding RNA SNHG1 in type 2 diabetes mellitus and its association with cell proliferation of pancreatic β-cell.

Background: To explore the association of long non-coding RNA (lncRNA) SNHG1/ miR-195 axis with type 2 diabetes mellitus (T2DM) and islet function.

Methods: The expression of SNHG1 and miR-195 was measured in T2DM patients and in healthy subjects. Correlation between indciators was evaluated using Pearson correlation analysis. INS-1 cells were used to perform the cell function assays. Insulin secretion by INS-1 was detected using ELISA. Cell counting kit-8 (CCK-8) and flow cytometry was used to detect cell proliferation and apoptosis. Luciferase report assay was to used to verify the target of SNHG1.

Results: The expression of SNHG1 was increased and miR-195 level was decreased in the serum of T2DM patients. Both SNHG1 and miR-195 could be biomarkers for T2DM diagnosis. The fasting plasma glucose (FPG) and HbA1c were positively related to SNHG1 and negatively related to miR-195. SNHG1 inhibited insulin secretion, and cell proliferation and promoted apoptosis of INS-1 cells via binding to miR-195.

Conclusions: Detection of SNHG1 and miR-195 might predict T2DM. SNHG1 could suppress proliferation and insulin secretion, but promote apoptosis of INS-1 cells via sponging miR-195.

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来源期刊

BMC Endocrine Disorders ENDOCRINOLOGY & METABOLISM-

CiteScore

4.40

自引率

0.00%

发文量

280

审稿时长

>12 weeks

期刊介绍： BMC Endocrine Disorders is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of endocrine disorders, as well as related molecular genetics, pathophysiology, and epidemiology.