基于冻干开啡尔牛奶的自纳米乳化系统通过微生物群-肠-脑轴对认知能力增强的影响

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Mai M Anwar, Amira A Boseila, Abeer A Mabrouk, Abdelfattah A Abdelkhalek, Amr Amin
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引用次数: 0

摘要

慢性炎症性肠病(IBD)的特点是肠道通透性改变,引发炎症、氧化应激和免疫因素。肠道微生物群紊乱通过双向肠脑轴影响大脑功能,通过炎症级联、氧化应激和神经递质水平影响行为。本研究强调了将冻干开菲尔牛奶单独作为固体载体和冻干开菲尔牛奶作为固体载体装载甘草提取物自纳米乳化自纳米悬浮液(SNESNS)对大鼠慢性 IBD 类诱导模型的潜在影响。将 30 毫克甘草提取物与 1 克所选 SNEDDS(30% 香芹油、60% 吐温 20 和 10%丙二醇(重量比))混合均匀后,制备甘草-SNESNS。甘草-SNESNS 与开菲尔牛奶混合,然后冷冻干燥。动态 TEM 图像和双峰粒度曲线证实,稀释后形成了双相纳米系统(纳米乳液和纳米悬浮液)。每天口服含 SNESNS(10 毫克/千克香芹油和 1 毫克/千克甘草)的冻干开菲尔牛奶(100 毫克/千克)可恢复诱导大鼠的正常体重和肠粘膜,同时显著减少粘膜下炎症细胞浸润。重要的是,与单独使用冻干开菲尔牛奶相比,这种疗法具有更佳的疗效,能更明显地缓解神经递质水平,改善记忆功能,从而解决肠脑轴紊乱问题。此外,它还使粪便微生物组成分、炎症细胞因子水平以及受检组织和血清中的氧化应激正常化。此外,每天服用含有开菲尔的 SNESNS 还能使疾病活动指数正常化,减轻 IBD 诱导的组织病理学变化,并部分恢复正常的肠道微生物群。这些变化与认知功能的改善有关,这归因于正常神经递质水平的维持以及诱发炎症因子和氧化应激水平的缓解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impact of Lyophilized Milk Kefir-Based Self-Nanoemulsifying System on Cognitive Enhancement via the Microbiota-Gut-Brain Axis.

Chronic inflammatory bowel disorders (IBDs) are characterized by altered intestinal permeability, prompting inflammatory, oxidative stress, and immunological factors. Gut microbiota disorders impact brain function via the bidirectional gut-brain axis, influencing behavior through inflammatory cascades, oxidative stress, and neurotransmitter levels. This study highlights the potential effect of integrating lyophilized milk kefir alone and lyophilized milk kefir as solid carriers loaded with a self-nanoemulsifying self-nanosuspension (SNESNS) of licorice extract on an induced chronic IBD-like model in rats. Licorice-SNESNS was prepared by the homogenization of 30 mg of licorice extract in 1 g of the selected SNEDDS (30% Caraway oil, 60% Tween 20, and 10% propylene glycol (w/w)). Licorice-SNESNS was mixed with milk kefir and then freeze-dried. Dynamic TEM images and the bimodal particle size curve confirmed the formation of the biphasic nanosystems after dilution (nanoemulsion and nanosuspension). Daily oral administration of lyophilized milk kefir (100 mg/kg) loaded with SNESNS (10 mg/kg Caraway oil and 1 mg/kg licorice) restored normal body weight and intestinal mucosa while significantly reducing submucosal inflammatory cell infiltration in induced rats. Importantly, this treatment demonstrated superior efficacy compared to lyophilized milk kefir alone by leading to a more significant alleviation of neurotransmitter levels and improved memory functions, thereby addressing gut-brain axis disorders. Additionally, it normalized fecal microbiome constituents, inflammatory cytokine levels, and oxidative stress in examined tissues and serum. Moreover, daily administration of kefir-loaded SNESNS normalized the disease activity index, alleviated histopathological changes induced by IBD induction, and partially restored the normal gut microbiota. These alterations are associated with improved cognitive functions, attributed to the maintenance of normal neurotransmitter levels and the alleviation of triggered inflammatory factors and oxidative stress levels.

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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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