{"title":"首次对黑海 Saccocoelium Cephali 幼虫阶段（Digenea: Haploporidae）进行现代形态学和分子描述。","authors":"Yuliya V. Belousova, Dmitry M. Atopkin","doi":"10.1007/s11686-024-00934-8","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p>Knowledge of haploporid trematode larvae is very poor. Until recently, only scattered reports from the Black and Medditeranean Seas were known. The present research is the first report of haploporid cercariae <i>S. cephali</i> from the gastropod <i>Hydrobia acuta</i> inhabiting the Black Sea. Thus, the present study aimed to investigate the larvae of one species from the Haploporidae family.</p><h3>Methods</h3><p><i>Hydrobia acuta</i> molluscs were collected in the estuary of the Chernaya River (the Black Sea) during 2011–2013 and examined for trematode larvae. Found parthenitae were drawn and preserved for DNA extractions. Morphological features were compared to nominal species, and molecular comparisons were made of the subunit ribosomal DNA with other Haploporidae species.</p><h3>Results</h3><p>The haploporid parthenitae were found in the gonads of <i>Hydrobia acuta</i> molluscs. Most of the morphological characteristics of the specimens investigated agree with those of <i>Saccococoelium </i>sp<i>.</i> and are closest to those of <i>S. cephali</i> by shape and length of the body, ratio of suckers, ratio of tail length to body, shape, and size of the pharynx. Phylogenetic analysis from our study generated with Bayesian algorithm showed that studied cercariae specimens from the Black Sea were within the hapolporine clade and closely related to <i>S. cephali</i> from Spain.</p><h3>Conclusion</h3><p>Morphological characteristics of cercariae emerging from <i>Hydrobia acuta</i> from the Black Sea and the analysis of partial 28S rDNA sequences support the conspecificity of the parasite with <i>S. cephali</i> mature worms from the Mediterranean Sea.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"1919 - 1925"},"PeriodicalIF":1.2000,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"First Modern Morphological and Molecular Description of Saccocoelium Cephali Larvae Stages (Digenea: Haploporidae) from the Black Sea\",\"authors\":\"Yuliya V. Belousova, Dmitry M. Atopkin\",\"doi\":\"10.1007/s11686-024-00934-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><p>Knowledge of haploporid trematode larvae is very poor. Until recently, only scattered reports from the Black and Medditeranean Seas were known. The present research is the first report of haploporid cercariae <i>S. cephali</i> from the gastropod <i>Hydrobia acuta</i> inhabiting the Black Sea. Thus, the present study aimed to investigate the larvae of one species from the Haploporidae family.</p><h3>Methods</h3><p><i>Hydrobia acuta</i> molluscs were collected in the estuary of the Chernaya River (the Black Sea) during 2011–2013 and examined for trematode larvae. Found parthenitae were drawn and preserved for DNA extractions. Morphological features were compared to nominal species, and molecular comparisons were made of the subunit ribosomal DNA with other Haploporidae species.</p><h3>Results</h3><p>The haploporid parthenitae were found in the gonads of <i>Hydrobia acuta</i> molluscs. Most of the morphological characteristics of the specimens investigated agree with those of <i>Saccococoelium </i>sp<i>.</i> and are closest to those of <i>S. cephali</i> by shape and length of the body, ratio of suckers, ratio of tail length to body, shape, and size of the pharynx. Phylogenetic analysis from our study generated with Bayesian algorithm showed that studied cercariae specimens from the Black Sea were within the hapolporine clade and closely related to <i>S. cephali</i> from Spain.</p><h3>Conclusion</h3><p>Morphological characteristics of cercariae emerging from <i>Hydrobia acuta</i> from the Black Sea and the analysis of partial 28S rDNA sequences support the conspecificity of the parasite with <i>S. cephali</i> mature worms from the Mediterranean Sea.</p></div>\",\"PeriodicalId\":6932,\"journal\":{\"name\":\"Acta Parasitologica\",\"volume\":\"69 4\",\"pages\":\"1919 - 1925\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2024-10-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Parasitologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11686-024-00934-8\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Parasitologica","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s11686-024-00934-8","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
目的：人们对单细胞吸虫幼虫的了解非常有限。直到最近，也只有来自黑海和地中海的零星报道。本研究首次报告了栖息在黑海的腹足类水蚤（Hydrobia acuta）中的单棘蚴笛笛蚴（S. cephali）。因此，本研究旨在调查 Haploporidae 科中一个物种的幼虫。方法：2011-2013 年期间，在切尔纳亚河（黑海）河口采集了 Hydrobia acuta 软体动物，并对其进行了吸虫幼虫检查。发现的部分虫体被提取并保存以用于 DNA 提取。将形态特征与标称物种进行比较，并将核糖体 DNA 亚单位与其他 Haploporidae 物种进行分子比较：结果：在 Acuta Hydrobia 软体动物的性腺中发现了单孔虫。调查标本的形态特征大多与 Saccocoelium sp.一致，在身体的形状和长度、吸盘的比例、尾长与身体的比例、咽部的形状和大小等方面与 S. cephali最为接近。利用贝叶斯算法进行的系统发育分析表明，所研究的黑海恙虫标本属于单孢子虫支系，与西班牙的 S. cephali 关系密切：结论：黑海 Acuta 水螅蚴的形态特征和部分 28S rDNA 序列分析表明，该寄生虫与地中海的 S. cephali 成熟蠕虫具有同种性。
First Modern Morphological and Molecular Description of Saccocoelium Cephali Larvae Stages (Digenea: Haploporidae) from the Black Sea
Purpose
Knowledge of haploporid trematode larvae is very poor. Until recently, only scattered reports from the Black and Medditeranean Seas were known. The present research is the first report of haploporid cercariae S. cephali from the gastropod Hydrobia acuta inhabiting the Black Sea. Thus, the present study aimed to investigate the larvae of one species from the Haploporidae family.
Methods
Hydrobia acuta molluscs were collected in the estuary of the Chernaya River (the Black Sea) during 2011–2013 and examined for trematode larvae. Found parthenitae were drawn and preserved for DNA extractions. Morphological features were compared to nominal species, and molecular comparisons were made of the subunit ribosomal DNA with other Haploporidae species.
Results
The haploporid parthenitae were found in the gonads of Hydrobia acuta molluscs. Most of the morphological characteristics of the specimens investigated agree with those of Saccococoelium sp. and are closest to those of S. cephali by shape and length of the body, ratio of suckers, ratio of tail length to body, shape, and size of the pharynx. Phylogenetic analysis from our study generated with Bayesian algorithm showed that studied cercariae specimens from the Black Sea were within the hapolporine clade and closely related to S. cephali from Spain.
Conclusion
Morphological characteristics of cercariae emerging from Hydrobia acuta from the Black Sea and the analysis of partial 28S rDNA sequences support the conspecificity of the parasite with S. cephali mature worms from the Mediterranean Sea.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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