冷凝物的光遗传学控制：原理与应用。

IF 4.7 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Molecular Biology Pub Date : 2024-10-24 DOI:10.1016/j.jmb.2024.168835

Zikang Dennis Huang , Lukasz J. Bugaj

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引用次数: 0

摘要

生物分子凝聚体出现在细胞生理和病理过程中，但凝聚体的具体作用或其动态往往难以确定。光遗传学提供了一个不断扩大的工具集来应对这些挑战，它提供了直接控制任意蛋白质凝集的工具，可以精确地控制它们在空间和时间上的形成、溶解和模式化。在这篇综述中，我们描述了光遗传学控制凝集的领域现状。我们调查了构成这一工具集基础的蛋白质及其衍生物，并讨论了区分它们的因素，以便为特定应用进行适当选择。我们还介绍了最近在基础研究和应用研究中使用光遗传缩聚技术的实例。最后，我们讨论了在设计用于光遗传凝聚的新蛋白质时需要考虑的重要设计因素，并展望了未来几年将进一步增强这一工具集的创新。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Optogenetic Control of Condensates: Principles and Applications

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Optogenetic Control of Condensates: Principles and Applications

Biomolecular condensates appear throughout cell physiology and pathology, but the specific role of condensation or its dynamics is often difficult to determine. Optogenetics offers an expanding toolset to address these challenges, providing tools to directly control condensation of arbitrary proteins with precision over their formation, dissolution, and patterning in space and time. In this review, we describe the current state of the field for optogenetic control of condensation. We survey the proteins and their derivatives that form the foundation of this toolset, and we discuss the factors that distinguish them to enable appropriate selection for a given application. We also describe recent examples of the ways in which optogenetic condensation has been used in both basic and applied studies. Finally, we discuss important design considerations when engineering new proteins for optogenetic condensation, and we preview future innovations that will further empower this toolset in the coming years.

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来源期刊

Journal of Molecular Biology 生物-生化与分子生物学

CiteScore

11.30

自引率

1.80%

发文量

412

审稿时长

28 days

期刊介绍： Journal of Molecular Biology (JMB) provides high quality, comprehensive and broad coverage in all areas of molecular biology. The journal publishes original scientific research papers that provide mechanistic and functional insights and report a significant advance to the field. The journal encourages the submission of multidisciplinary studies that use complementary experimental and computational approaches to address challenging biological questions. Research areas include but are not limited to: Biomolecular interactions, signaling networks, systems biology; Cell cycle, cell growth, cell differentiation; Cell death, autophagy; Cell signaling and regulation; Chemical biology; Computational biology, in combination with experimental studies; DNA replication, repair, and recombination; Development, regenerative biology, mechanistic and functional studies of stem cells; Epigenetics, chromatin structure and function; Gene expression; Membrane processes, cell surface proteins and cell-cell interactions; Methodological advances, both experimental and theoretical, including databases; Microbiology, virology, and interactions with the host or environment; Microbiota mechanistic and functional studies; Nuclear organization; Post-translational modifications, proteomics; Processing and function of biologically important macromolecules and complexes; Molecular basis of disease; RNA processing, structure and functions of non-coding RNAs, transcription; Sorting, spatiotemporal organization, trafficking; Structural biology; Synthetic biology; Translation, protein folding, chaperones, protein degradation and quality control.