细胞外囊泡相关 DNA 的检测、生物功能和液体活检应用。

IF 9.5 2区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
Shan Guo, Xin Wang, Danni Shan, Yu Xiao, Lingao Ju, Yi Zhang, Gang Wang, Kaiyu Qian
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引用次数: 0

摘要

细胞衍生的细胞外囊泡(EVs)携带多种生物大分子,如核酸、蛋白质、代谢物和脂质,反映了它们的来源细胞,在生理和病理条件下都会释放出来。研究表明,EV 可介导细胞间的交流,并可作为生物标记物。EV相关DNA(EV-DNA)包括基因组DNA和线粒体DNA(即gDNA和mtDNA)片段。一些研究显示,EV-DNA 可以代表亲代细胞的全部核基因组和线粒体基因组。此外,载入EV的DNA片段是稳定的,可以转移到受体细胞中调节其生物功能。在这篇综述中,我们总结并讨论了 EV-DNA 的研究进展,重点是群体-EV 和单体-EV 水平的 EV-DNA 检测、基因转移相关的生物功能以及作为疾病液体活检生物标记物的临床应用。我们希望这篇综述能为未来的 EV-DNA 研究提供潜在的方向或指导。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The detection, biological function, and liquid biopsy application of extracellular vesicle-associated DNA.

Cell-derived extracellular vesicles (EVs), which carry diverse biomolecules such as nucleic acids, proteins, metabolites, and lipids reflecting their cell of origin, are released under both physiological and pathological conditions. EVs have been demonstrated to mediate cell-to-cell communication and serve as biomarkers. EV-associated DNA (EV-DNA) comprises genomic and mitochondrial DNA (i.e., gDNA and mtDNA) fragments. Some studies have revealed that EV-DNA can represent the full nuclear genome and mitochondrial genome of parental cells. Furthermore, DNA fragments loaded into EVs are stable and can be transferred to recipient cells to regulate their biological functions. In this review, we summarized and discussed EV-DNA research advances with an emphasis on EV-DNA detection at the population-EV and single-EV levels, gene transfer-associated biological functions, and clinical applications as biomarkers for disease liquid biopsy. We hope that this review will provide potential directions or guidance for future EV-DNA investigations.

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来源期刊
Biomarker Research
Biomarker Research Biochemistry, Genetics and Molecular Biology-Molecular Medicine
CiteScore
15.80
自引率
1.80%
发文量
80
审稿时长
10 weeks
期刊介绍: Biomarker Research, an open-access, peer-reviewed journal, covers all aspects of biomarker investigation. It seeks to publish original discoveries, novel concepts, commentaries, and reviews across various biomedical disciplines. The field of biomarker research has progressed significantly with the rise of personalized medicine and individual health. Biomarkers play a crucial role in drug discovery and development, as well as in disease diagnosis, treatment, prognosis, and prevention, particularly in the genome era.
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