使用 DROPPS 生成高保真蛋白质组图谱的程序。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-10-18 DOI:10.1016/j.xpro.2024.103397

Matthew Waas, Meinusha Govindarajan, Amanda Khoo, Charlotte Zuo, Aastha Aastha, Jilin He, Michael Woolman, Annie Ha, Brian Lin, Thomas Kislinger

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引用次数: 0

摘要

基于深度质谱的稀有细胞群蛋白质组图谱分析一直受到样品输入要求的限制。在这里，我们介绍了一种基于液滴的蛋白质组样品一锅制备（DROPPS）方案，这是一种易于使用的低投入平台，可生成 100-2,500 个细胞的高保真蛋白质组图谱。我们介绍了在同一微升液滴孔中沉积细胞材料、细胞裂解和消化蛋白质的步骤。我们预计 DROPPS 将加速多种稀有细胞群的生物学驱动蛋白质组学研究。有关使用和执行该方案的完整细节，请参阅 Waas 等人的文章1。

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Protocol for generating high-fidelity proteomic profiles using DROPPS.

Deep mass spectrometry-based proteomic profiling of rare cell populations has been constrained by sample input requirements. Here, we present a protocol for droplet-based one-pot preparation for proteomic samples (DROPPS), an accessible low-input platform that generates high-fidelity proteomic profiles of 100-2,500 cells. We describe steps for depositing cellular material, cell lysis, and digesting proteins in the same microliter-droplet well. We anticipate DROPPS will accelerate biology-driven proteomic research for a multitude of rare cell populations. For complete details on the use and execution of this protocol, please refer to Waas et al.¹.

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