茄子潜伏病毒位于茄子感染细胞的叶绿体和细胞核中。

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2024-10-15 DOI:10.1186/s12985-024-02530-8

Marcelo Eiras, Verónica Aragonés, Jorge Marqués, María Dolores Gómez, José-Antonio Daròs

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引用次数: 0

摘要

属于 Avsunviroid 和 Pelamovirod 属（Avsunviroidae 科）的病毒复制并积累在受感染细胞的叶绿体中。在本报告中，我们通过使用地高辛-UTP 标记的核糖体进行 RNA 原位杂交证实，茄潜伏病毒（ELVd）的阳性链（茄潜伏病毒是 Avsunviroidae 科中 Elaviroid 属的唯一成员）也会在感染细胞的叶绿体中积累。然而，将 ELVd 的原位杂交信号与真正的叶绿体和核非编码 RNA（如叶绿体 5S rRNA 和 U1 小核 RNA）的信号进行比较后发现，这种病毒也存在于受感染细胞的细胞核中。这些结果表明，Avsunviroidae 科病毒的亚细胞定位可能比以前假定的更为复杂，在感染周期中会动态地存在于多个区室中。

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Eggplant latent viroid is located in the chloroplasts and nuclei of eggplant infected cells.

Viroids that belong to genera Avsunviroid and Pelamovirod (family Avsunviroidae) replicate and accumulate in the chloroplasts of infected cells. In this report, we confirmed by RNA in situ hybridization using digoxigenin-UTP-labelled riboprobes that the positive strands of eggplant latent viroid (ELVd), the only member of genus Elaviroid within the family Avsunviroidae, also accumulate in the chloroplasts of infected cells. However, comparison of ELVd in situ hybridization signals with those from bona fide chloroplastic and nuclear non-coding RNAs, such as chloroplast 5S rRNA and U1 small nuclear RNA, supports the notion that this viroid is also present in the nuclei of infected cells. These results suggest that the subcellular localization of viroids within the family Avsunviroidae may be more complex than previously assumed with dynamic presence in several compartments during the infectious cycle.

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.