通过免疫组化和超微结构鉴定婴儿血管瘤中的毛细血管细胞。

IF 1.1 4区 医学 Q4 MICROSCOPY
Ultrastructural Pathology Pub Date : 2024-11-01 Epub Date: 2024-10-13 DOI:10.1080/01913123.2024.2415608
Han-Wen Ding, Qian Wang, Min Wang, Yong Chen, Si-Ming Yuan
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引用次数: 0

摘要

端细胞(TC)是多种肿瘤基质微环境中的一种独特细胞实体;迄今为止,它们在婴儿血管瘤(IH)中的存在几乎仍未得到研究。因此,本研究通过免疫组织化学、免疫荧光共聚焦显微镜和透射电子显微镜等方法,对婴幼儿血管瘤中端粒细胞的免疫表型、位置、形态和超微结构进行了表征。通过CD34、PDGFR-α、Vimentin和AQP-1免疫染色初步确定了端粒细胞。用AQP-1/CD31、AQP-1/Glut-1、AQP-1/α-SMA、AQP-1/CD146和AQP-1/CD133双重免疫荧光分析IH中端粒细胞、干细胞、内皮细胞和周细胞之间的空间关系。IH 中 TC 的 CD31、Glut-1、CD146、α-SMA、CD133 和 C-kit 免疫阴性。超微结构检查证实了TC的存在,即具有特征性胞质突起(即端突)的基质细胞,它们在微血管周围形成迷宫般的网络,并释放细胞外囊泡。我们的研究提供了端粒细胞存在的证据,而 PDGFR-α 和 AQP-1 是 IH 的特异性抗原标记。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunohistochemical and ultrastructural identification of telocytes in the infantile hemangioma.

Telocytes (TCs) are a distinctive cell entity of the stromal microenvironment of multiple tumors; to date, their existence in infantile hemangioma (IH) remains almost unexplored. This study was therefore undertaken to characterize the immunophenotype, location, morphology, and ultrastructure of telocytes in the IH by means of immunohistochemistry, immunofluorescence confocal microscopy, and transmission electron microscopy. Telocytes were initially identified by CD34, PDGFR-α, Vimentin, and AQP-1 immunostaining. Analyzing the spatial relationship among telocytes, stem cells, endothelial cells, pericytes in the IH with AQP-1/CD31, AQP-1/Glut-1, AQP-1/α-SMA, AQP-1/CD146 and AQP-1/CD133 double immunofluorescence. TCs were immunonegative for CD31, Glut-1, CD146, α-SMA, CD133, and C-kit in the IH. The ultrastructural examination confirmed the presence of TCs, namely stromal cells with characteristic cytoplasmic processes (i.e. telopodes) forming labyrinthine networks around microvessels and releasing extracellular vesicles. Our study provides evidence that telocytes are present and PDGFR-α and AQP-1 are specific antigenic markers in the IH.

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来源期刊
Ultrastructural Pathology
Ultrastructural Pathology 医学-病理学
CiteScore
2.00
自引率
10.00%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.
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