全基因组 RNA-Seq 鉴定出 TP53 介导的胚胎干细胞抑制肿瘤侵袭和转移。

IF 7.1 2区医学 Q1 CELL & TISSUE ENGINEERING

Stem Cell Research & Therapy Pub Date : 2024-10-16 DOI:10.1186/s13287-024-04000-y

Yatong Li, Yongna Fan, Yunyi Xie, Limin Li, Juan Li, Jingyi Liu, Zhengyu Jin, Huadan Xue, Zhiwei Wang

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引用次数: 0

摘要

胚胎干细胞介导杀瘤活性的发现揭示了胚胎干细胞与肿瘤细胞之间的密切关系，但人们对胚胎干细胞在肿瘤进展中的功能作用还知之甚少。为了进一步研究肿瘤细胞与造血干细胞之间的相互作用，我们将小鼠造血干细胞与小鼠胰腺癌Pan02细胞或小鼠黑色素瘤B16-F10细胞在Transwell中共同培养，发现造血干细胞能显著抑制肿瘤细胞的侵袭。将干细胞应用于肿瘤小鼠体内可明显抑制肿瘤的转移。对肿瘤细胞和间充质干细胞共培养物的RNA-Seq分析发现，TP53和相关信号传导是参与间充质干细胞介导的肿瘤细胞侵袭和转移抑制的主要通路，这表明间充质干细胞治疗癌症具有潜在的临床应用前景。

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Genome-wide RNA-Seq identifies TP53-mediated embryonic stem cells inhibiting tumor invasion and metastasis.

The discovery of embryonic stem cell (ESC) mediating tumoricidal activity revealed the intimate relationship between ESCs and tumor cells, but the functional role of ESCs in tumor progression is poorly understood. To further investigate tumor cell and ESC interactions, we co-cultured mouse ESCs with mouse pancreatic cancer Pan02 cells or mouse melanoma B16-F10 cells in Transwell, and found that tumor cell invasion was significantly inhibited by ESCs. Application of ESCs to tumor-bearing mice resulted in significant inhibition of tumor metastasis in vivo. RNA-Seq analyses of tumor cell and ESC co-cultures identified TP53 and related signalling as major pathways involved in ESC-mediated inhibition of tumor cell invasion and metastasis, which indicated the potential clinical application of ESCs to treat cancer.

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来源期刊

Stem Cell Research & Therapy CELL BIOLOGY-MEDICINE, RESEARCH & EXPERIMENTAL

CiteScore

13.20

自引率

8.00%

发文量

525

审稿时长

1 months

期刊介绍： Stem Cell Research & Therapy serves as a leading platform for translational research in stem cell therapies. This international, peer-reviewed journal publishes high-quality open-access research articles, with a focus on basic, translational, and clinical research in stem cell therapeutics and regenerative therapies. Coverage includes animal models and clinical trials. Additionally, the journal offers reviews, viewpoints, commentaries, and reports.