使用全自动细菌检测设备 RAISUS S4 检测日本耐碳青霉烯革兰阴性杆菌。

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES
Yumiko Funashima, Rin Hamabe, Kei Tominaga, Kentaro Wakamatsu, Takahiro Yaguchi, Zenzo Nagasawa, Tsukuru Umemura
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引用次数: 0

摘要

目的我们研究了一种使用美罗培南(MEPM)快速检测产碳青霉烯酶革兰阴性杆菌(CP-GNR)的方法,以评估抗菌药敏感性检测的效率:方法:我们利用耐药菌监测功能(RAISUS S4)监测生长曲线。在 RAISUS 18 小时法(18-h 法)和 RAISUS 快速法(快速法)两种抗菌药敏感性检测中,使用 RAISUS S4 对肠杆菌(F-GNR)和非发酵革兰氏阴性杆菌(NF-GNR)进行了 CP-GNR 的快速检测:当 F-GNR 的 MEPM MIC ≥ 0.25 μg/mL 时,CP-GNR 的检测灵敏度为:18 h 法 100%(58/58),快速法 98.3%(57/58);最短检测时间分别为 5.3 和 4.0 h。当 NF-GNR 基于 MEPM MIC > 8 μg/mL 时,两种方法检测 CP-GNR 的灵敏度均为 100%(58/58)。此外,在使用 18 h 方法监测耐药菌的分析中,当 F-GNR 的筛选浓度≥ 2 µg/mL 时,约有 50%的耐药基因型(NDM、GES 和 KPC)在约 7 h 内被检测出来,但检测 IMP 和 VIM 则需要 11-12 h:RAISUS S4 的 18 小时快速方法与 CLSI 微稀释法的结果高度相关,使用 RAISUS S4 监测生长曲线的功能可快速检测 CP-GNR。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection of carbapenem-resistant gram-negative bacilli in Japan using the fully automated bacterial testing device RAISUS S4.

Objective: We investigated a rapid detection method for carbapenemase-producing gram-negative bacilli (CP-GNR) using meropenem (MEPM) to assess the efficiency of the antimicrobial susceptibility testing.

Methods: We used the function that can monitor the growth curve with the resistant bacteria monitoring function (RAISUS S4). Rapid detection of CP-GNR was performed using RAISUS S4 in two types of antimicrobial susceptibility testing, the RAISUS 18-hour method (18-h method) and RAISUS rapid method (rapid method) for Enterobacterales (F-GNR) and non-fermenting Gram-negative bacilli (NF-GNR).

Results: When F-GNR were based on MEPM MIC ≥ 0.25 μg/mL, CP-GNR were detected with a sensitivity of 100% (58/58) for the 18-h method and 98.3% (57/58) for the rapid method; the shortest detection times were 5.3 and 4.0 h, respectively. When NF-GNR were based on MEPM MIC > 8 μg/mL, it was possible to detect CP-GNR with 100% sensitivity (58/58) in both methods. Furthermore, in the analysis using the 18-h method for monitoring resistant bacteria, when ≥ 2 µg/mL was used as the screening concentration for F-GNR, approximately 50% of the resistant genotypes, NDM, GES, and KPC, were detected in approximately 7 h. However, detecting the IMP and VIM took 11-12 h.

Conclusions: The 18-h and rapid methods with RAISUS S4 were highly correlated with the results of the microdilution method of CLSI, and CP-GNR detection was rapid using a function that can monitor the growth curve with RAISUS S4.

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来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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