含有牛膝和Trachyspermum ammi的多草本漱口水对特定牙周病原体的抗菌和细胞毒性特性。

IF 1.7 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Ayurveda and Integrative Medicine Pub Date : 2024-09-01 DOI:10.1016/j.jaim.2024.101046

Ram Surath Kumar , Anil V. Ankola , Mahantesh B. Nagamoti , Roopali M. Sankeshwari , Kishori P. Sutar , Shushant I. Jigan , Atrey J. Pai Khot

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引用次数: 0

摘要

背景：洗必泰（CHX）因其抗菌功效和在化学牙菌斑控制方面的有效性而被视为黄金标准。然而，长期使用会产生一些不良反应。Achyranthes aspera 和 Trachyspermum ammi 等草药已在许多临床病例中使用，在牙周疾病的治疗中，它们似乎是 CHX 的重要替代品：本体外研究旨在评估和比较含有香紫苏和安米的新型多草本漱口水与 0.2% CHX 漱口水对牙龈卟啉单胞菌、连翘菌和放线菌的抗菌潜力和细胞毒性作用：用索氏提取器法制备了 A. aspera 和 T. ammi 的乙醇提取物，并对其进行了初步的植物化学筛选。以 1:1、2:1、1:2（w/v）的比例分别评估了天南星和安米的单个植物提取物和植物提取物混合物（PEM）对选定牙周病原体的最小抑菌浓度（雷沙唑啉微滴定法）和最小杀菌浓度（涂布平板法），并与 CHX 进行了比较。评估了多草本漱口水对成人牙龈成纤维细胞的抑制区（井扩散法）和细胞毒性（MTT 法）。所有实验均一式三份：结果：PEMs 和多草本漱口水对测试的牙周病原体具有明显的抗菌活性，与 CHX 不相上下。细胞毒性试验结果表明，多草本漱口水中存活细胞的比例为 82.1%，证明其具有良好的生物相容性：结论：与 CHX 相比，A. aspera 和 T. ammi 漱口水对牙周病原体具有相当的抗菌活性。

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Antibacterial and cytotoxicity properties of a polyherbal mouthwash containing Achyranthes aspera and Trachyspermum ammi against selected periodontal pathogens

Background

Chlorhexidine (CHX) is considered as a gold standard for its antibacterial efficacy and substantivity in chemical plaque control. However, some adverse effects are associated with its prolonged use. Herbal medicines like Achyranthes aspera and Trachyspermum ammi have been used in many clinical conditions, and they appear to be a valuable substitute next to CHX in the management of periodontal diseases.

Objective

This in vitro study was designed to assess and compare the antibacterial potential and cytotoxic effects of novel polyherbal mouthwash containing A. aspera and T. ammi with 0.2% CHX mouthwash against Porphyromonas gingivalis, Tannerella forsythia, and Aggregatibacter actinomycetemcomitans.

Methods

Ethanolic extracts of A. aspera and T. ammi were prepared by the Soxhlet apparatus method and were subjected to preliminary phytochemical screening. The individual plant extracts and the plant extract mixture (PEM) of A. aspera and T. ammi in the ratio of 1:1, 2:1, 1:2 (w/v) were assessed for minimum inhibitory concentration (resazurin microtitre assay) and minimum bactericidal concentration (spread plating method) against selected periodontal pathogens in comparison to CHX. The polyherbal mouthwash was assessed for zone of inhibition (well diffusion method) and cytotoxicity (MTT assay) on adult human gingival fibroblasts. All the experiments were performed in triplicate.

Results

The antibacterial activity was evident in the PEMs, and polyherbal mouthwash against tested periodontal pathogens and was comparable to CHX. The cytotoxicity assay findings confirmed that polyherbal mouthwash exhibited 82.1% of surviving cells which proved good biocompatibility.