初步评估 rEi-SAG19 对兔子肠道埃默氏菌感染的保护作用。

IF 1.4 4区医学 Q3 PARASITOLOGY

Experimental parasitology Pub Date : 2024-10-17 DOI:10.1016/j.exppara.2024.108845

Jiayan Pu, Ge Hao, Hao Chen, Wei He, Changming Xiong, Jie Xiao, Guangyou Yang

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引用次数: 0

摘要

肠道埃米拉氏菌是家兔致病性最强的球虫之一。目前，抗球虫药物治疗是控制兔球虫病的主要措施，但存在耐药性和药物残留问题。因此，疫苗被用作一种替代策略。呼吸复合原虫的表面抗原（SAGs）在粘附和侵入宿主肠道细胞中发挥作用，被认为是疫苗的潜在候选抗原。本研究对5个Ei-SAGs基因的四个发育阶段进行了转录分析，然后筛选出了原核表达的Ei-SAG19基因，并通过免疫印迹法研究了重组SAG19（rEi-SAG19）的反应原性。为了评估rEi-SAG19的保护作用，将兔子（n = 40）随机分为四组（空白对照组、PBS感染组、Trx-His-S-Quil-A感染组和rEi-SAG19免疫组），rEi-SAG19免疫组在颈部皮下注射100 μg rEi-SAG19，间隔两周，第二次免疫两周后用5×104同源卵囊进行挑战。挑战两周后，所有兔子均被处死。之后，每周检测血清特异性 IgG 抗体的水平，并测定挑战前血清中细胞因子的水平。实验结束后，计算兔子的体重增加、卵囊减少率、病变评分和抗球虫指数（ACI）。结果表明，rEi-SAG19 具有良好的反应生成性。rEi-SAG19免疫组兔子的相对增重率、卵囊减少率和ACI分别为80.51%、72.6%和165.1，具有中等保护作用。血清特异性 IgG 抗体和 IL-4 水平显著升高（P < 0.05），但 IL-2、IFN-γ 和 IL-10 水平无显著差异（P > 0.05）。我们的研究结果表明，rEi-SAG19 对家兔肠道埃希氏菌感染有中度保护作用（ACI = 165.1）。因此，rEi-SAG19 可用作肠道埃希氏菌疫苗的候选抗原。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Preliminary evaluation of the protective effect of rEi-SAG19 on Eimeria intestinalis infection in rabbits

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Preliminary evaluation of the protective effect of rEi-SAG19 on Eimeria intestinalis infection in rabbits

Eimeria intestinalis is one of the most pathogenic coccidia species in rabbits. Anticoccidial treaments are the main measures to control rabbit coccidiosis now, but there are drug resistance and residues concerns. Therefore, vaccine has been used as an alternative strategy. The surface antigens (SAGs) of apicomplexan protozoa play a role in adhesion and invasion of host intestinal cells, and are considered to be potential candidate antigens for vaccines. In this study, transcriptional analysis of 5 Ei-SAGs genes at four developmental stages was conducted, then the Ei-SAG19 gene were screened out for prokaryotic expression and the reactogenicity of recombinant SAG19 (rEi-SAG19) was investigated by immunoblotting. To assessment the protective effects of rEi-SAG19, rabbits (n = 40) were randomly divided into four groups (Blank control, PBS-infected, Trx-His-S-Quil-A-infected and rEi-SAG19 immunized groups), the rEi-SAG19 immunized group was subcutaneously immunized with 100 μg rEi-SAG19 in the neck with an interval of two weeks, and challenged with 5 × 10⁴ homologous oocysts two weeks after the second immunization. Two weeks after the challenge, all rabbits were sacrificed. After that, the level of serum specific IgG antibody was detected weekly and the level of cytokines in serum before the challenge were determined. At the end of the experiment, the weight gain, oocyst reduction rate, lesion score and anticoccidial index (ACI) were calculated. The results showed that rEi-SAG19 has a good reactogenicity. The relative weight gain rate, oocyst reduction rate and ACI of the rabbits in rEi-SAG19 immunized group were 80.51%, 72.6%, and 165.1, respectively, which has a moderate protective effect. The level of serum specific IgG antibody and IL-4 rised significantly (P < 0.05), but the levels of IL-2, IFN-γ and IL-10 had no significant difference (P > 0.05). Our results indicated that rEi-SAG19 could provides moderate protective effect against E. intestinalis infection in rabbits (ACI = 165.1). Therefore, rEi-SAG19 could be used as a vaccine candidate antigen for E. intestinalis.

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来源期刊

Experimental parasitology 医学-寄生虫学

CiteScore

3.10

自引率

4.80%

发文量

160

审稿时长

3 months

期刊介绍： Experimental Parasitology emphasizes modern approaches to parasitology, including molecular biology and immunology. The journal features original research papers on the physiological, metabolic, immunologic, biochemical, nutritional, and chemotherapeutic aspects of parasites and host-parasite relationships.