{"title":"用于伤口愈合检测的不同宽度的实验装置。","authors":"Wen Jia, Junbao Du, Yun He, Shuyu Fang, Yang Bi","doi":"10.1159/000542051","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Cellular wound healing assay is an important experimental technique for detecting cell migration in vitro. Scratching on monolayer cells using a pipette tip is commonly used. However, it is difficult to guarantee the scratch with the same width, and the initial scratch width has a large impact on the experimental results for different treatment factors or different cell types. To optimize this assay for diverse experimental requirements, we developed an experimental device capable of generating scratches with variable widths.</p><p><strong>Methods: </strong>Our device offers the flexibility of selecting among four widths to create cell scratches, enabling the choice of an optimal initial scratch width for specific cell types and experimental conditions.</p><p><strong>Results: </strong>This device produced straight, clean wounds with precise widths. Comparing cell growth in the four width wounds, Hepa1-6 and HUMSCs showed the greatest difference in 0.6 cm wound, 143B at 0.9 cm wound and urine-derived stem cells at 1.2 cm wound were significantly different, which suggests that the width of the wounds has a huge impact on the experimental results. Compared to other wound inserts on the market, our device is more efficient and economical.</p><p><strong>Conclusion: </strong>This versatile and practical device provides a valuable solution for studying cell migration, facilitating a deeper understanding of cellular behaviors and the development of therapeutic strategies.</p>","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":" ","pages":"1-9"},"PeriodicalIF":2.9000,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"An Experimental Device with Different Widths for Wound Healing Assay.\",\"authors\":\"Wen Jia, Junbao Du, Yun He, Shuyu Fang, Yang Bi\",\"doi\":\"10.1159/000542051\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Cellular wound healing assay is an important experimental technique for detecting cell migration in vitro. Scratching on monolayer cells using a pipette tip is commonly used. However, it is difficult to guarantee the scratch with the same width, and the initial scratch width has a large impact on the experimental results for different treatment factors or different cell types. To optimize this assay for diverse experimental requirements, we developed an experimental device capable of generating scratches with variable widths.</p><p><strong>Methods: </strong>Our device offers the flexibility of selecting among four widths to create cell scratches, enabling the choice of an optimal initial scratch width for specific cell types and experimental conditions.</p><p><strong>Results: </strong>This device produced straight, clean wounds with precise widths. Comparing cell growth in the four width wounds, Hepa1-6 and HUMSCs showed the greatest difference in 0.6 cm wound, 143B at 0.9 cm wound and urine-derived stem cells at 1.2 cm wound were significantly different, which suggests that the width of the wounds has a huge impact on the experimental results. Compared to other wound inserts on the market, our device is more efficient and economical.</p><p><strong>Conclusion: </strong>This versatile and practical device provides a valuable solution for studying cell migration, facilitating a deeper understanding of cellular behaviors and the development of therapeutic strategies.</p>\",\"PeriodicalId\":9717,\"journal\":{\"name\":\"Cells Tissues Organs\",\"volume\":\" \",\"pages\":\"1-9\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-10-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cells Tissues Organs\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1159/000542051\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ANATOMY & MORPHOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cells Tissues Organs","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1159/000542051","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}
An Experimental Device with Different Widths for Wound Healing Assay.
Introduction: Cellular wound healing assay is an important experimental technique for detecting cell migration in vitro. Scratching on monolayer cells using a pipette tip is commonly used. However, it is difficult to guarantee the scratch with the same width, and the initial scratch width has a large impact on the experimental results for different treatment factors or different cell types. To optimize this assay for diverse experimental requirements, we developed an experimental device capable of generating scratches with variable widths.
Methods: Our device offers the flexibility of selecting among four widths to create cell scratches, enabling the choice of an optimal initial scratch width for specific cell types and experimental conditions.
Results: This device produced straight, clean wounds with precise widths. Comparing cell growth in the four width wounds, Hepa1-6 and HUMSCs showed the greatest difference in 0.6 cm wound, 143B at 0.9 cm wound and urine-derived stem cells at 1.2 cm wound were significantly different, which suggests that the width of the wounds has a huge impact on the experimental results. Compared to other wound inserts on the market, our device is more efficient and economical.
Conclusion: This versatile and practical device provides a valuable solution for studying cell migration, facilitating a deeper understanding of cellular behaviors and the development of therapeutic strategies.
期刊介绍:
''Cells Tissues Organs'' aims at bridging the gap between cell biology and developmental biology and the emerging fields of regenerative medicine (stem cell biology, tissue engineering, artificial organs, in vitro systems and transplantation biology). CTO offers a rapid and fair peer-review and exquisite reproduction quality. Special topic issues, entire issues of the journal devoted to a single research topic within the range of interests of the journal, are published at irregular intervals.