用不同溶剂提取的Ajuga integrifolia叶提取物的体外抗氧化和抗菌活性。

IF 3.3 2区 医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE
Workineh Mengesha Fereja, Wakuma Debessa Geleta, Engida Desalegn, Mitiku Moaneanda, Adugna Abdissa, Firehiwot Belyneh Usamo
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引用次数: 0

摘要

背景:由于耐多药细菌的发病率不断上升,许多疾病日益成为全球公认的公共卫生问题。最近,人们对利用本地药用植物治疗传染性疾病的兴趣日益浓厚,这凸显了寻找新的生物活性植物化学物质的必要性。Ajuga integrifolia 是一种常用于治疗多种疾病的传统药物,但其有效性尚未得到科学验证。本研究旨在评估通过不同溶剂系统生产的 A. integrifolia 叶提取物的总酚和类黄酮含量,并评估其生物活性:方法:采用索氏提取法从不同溶剂(甲醇、乙醇和水)中获得粗提取物。采用 2,2-二苯基-1-苦基肼(DPPH)和铁还原力检测法测定其抗氧化活性、通过琼脂盘扩散技术,根据提取物对两种革兰氏阴性菌(大肠杆菌(ATCC-25922)和铜绿假单胞菌(ATCC-43495))和两种革兰氏阳性菌(金黄色葡萄球菌(ATCC-25923)和化脓性链球菌(ATCC-19615))的最小抑菌浓度(MIC)来评估提取物的抗菌活性。结果:所有提取物中都含有大量的总酚含量(TPC)和类黄酮含量(TFC)。在所有试验中,提取物都具有很强的抗氧化活性。盘扩散和 MIC 结果表明,在 3.125 mg/mL 的浓度下,A. integrifolia 叶片的甲醇和乙醇提取物能够抑制金黄色葡萄球菌的生长。然而,水提取物对大肠杆菌和绿脓杆菌无效:这些研究结果表明,A. integrifolia 叶提取物具有合理的生物活性。这些发现强调了集成叶作为健康益处来源的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro antioxidant and antibacterial activities of Ajuga integrifolia leaf extracts obtained with different solvents.

Background: Many diseases are increasingly recognized as public health concerns worldwide because of the increasing incidence of multidrug-resistant bacteria. Recently, interest in the use of indigenous medicinal plants to treat infectious illnesses has increased, highlighting the need to find new bioactive phytochemicals. Ajuga integrifolia is a plant commonly utilized in traditional drugs to treat a wide range of diseases, although its effectiveness has not been scientifically validated. The present study aimed to evaluate the total phenolic and flavonoid contents and assess the biological activities of A. integrifolia leaf extracts produced via different solvent systems.

Methods: Soxhlet extraction was employed to obtain crude extracts from different solvents (methanol, ethanol and water). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric-reducing power assays were used to measure the antioxidant activity, and the antibacterial activity of the extract was evaluated on the basis of its minimum inhibitory concentration (MIC) against two gram-negative bacteria (Escherichia coli (ATCC-25922) and Pseudomonas aeruginosa (ATCC-43495)) and two gram-positive bacteria (Staphylococcus aureus (ATCC-25923) and Streptococcus pyogenes (ATCC-19615)) via the agar disk-diffusion technique.

Results: A significant amount of total phenolic content (TPC) and flavonoid content (TFC) were present in all the extracts. The extracts presented powerful antioxidant activity in all the assays. The disc diffusion and MIC results revealed the ability of the methanol and ethanol extracts of A. integrifolia leaves to inhibit S. aureus growth at a concentration of 3.125 mg/mL. However, the water extracts were ineffective against E. coli and P. aeruginosa.

Conclusions: These findings indicate that A. integrifolia leaf extracts have reasonable biological activities. These findings underscore the importance of A. integrifolia leaves as a source of health benefits.

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来源期刊
BMC Complementary Medicine and Therapies
BMC Complementary Medicine and Therapies INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
6.10
自引率
2.60%
发文量
300
审稿时长
19 weeks
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