Niloofar Sadeghi, Aaryn Mustoe, Corinna N Ross, John R McCarrey, Brian P Hermann
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Here, we identify characteristics of high-quality semen samples and validate a simple method for selecting high-quality sperm.</p><p><strong>Methods: </strong>Computer-assisted sperm analysis (CASA) was used to evaluate sperm quality in semen samples collected from 44 marmosets and assessed the use of the swim-up method for the selection of high-quality sperm was also tested in half the samples as a potential means to optimize in vitro fertilization or intrauterine insemination.</p><p><strong>Results: </strong>For each reference parameter, samples at or below the 5th percentile were categorized as abnormal sperm, while those above the 5th percentile were considered to be normal. Among normal samples, those at or above the 50th percentile were categorized as high-quality. High-quality semen samples exhibited the following characteristics: semen volume ≥ 30 µL; sperm count ≥ 10<sup>7</sup>/ejaculate; total motility ≥ 35%; and normal morphology ≥ 5%. Sperm isolated by swim-up exhibited superior sperm progressive motility (19.7% ± 4.5 vs. 5.6% ± 2.1; P = 0.01) and normal morphology (13.1 ± 1.59 vs. 7.65 ± 1.1; P < 0.001) compared with unselected sperm.</p><p><strong>Conclusion: </strong>This study defines robust, statistically supported reference values for evaluating marmoset semen samples to assist with the identification of optimal sperm donors and the selection of high-quality sperm samples for assisted reproduction. Ultimately, these reference values combined with a validated selection method will contribute to consistent standards for the international sharing of genetically diverse and/or gene-edited marmoset sperm for research and reproduction.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Benchmarks defining high-quality sperm in the common marmoset (Callithrix jacchus).\",\"authors\":\"Niloofar Sadeghi, Aaryn Mustoe, Corinna N Ross, John R McCarrey, Brian P Hermann\",\"doi\":\"10.1111/andr.13782\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Common marmosets (Callithrix jacchus) are increasingly recognized as valuable nonhuman primates (NHPs) for biomedical research due to their small size and short reproductive cycle and lifespan relative to other NHP species. Maximizing the utility of captive research marmosets, including genetically manipulated animals, will require the use of assisted reproductive techniques (ART) including manipulation, storage, and sharing of marmoset sperm. Here, we identify characteristics of high-quality semen samples and validate a simple method for selecting high-quality sperm.</p><p><strong>Methods: </strong>Computer-assisted sperm analysis (CASA) was used to evaluate sperm quality in semen samples collected from 44 marmosets and assessed the use of the swim-up method for the selection of high-quality sperm was also tested in half the samples as a potential means to optimize in vitro fertilization or intrauterine insemination.</p><p><strong>Results: </strong>For each reference parameter, samples at or below the 5th percentile were categorized as abnormal sperm, while those above the 5th percentile were considered to be normal. 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引用次数: 0
摘要
背景:普通狨猴(Callithrix jacchus)由于体型小、繁殖周期短、寿命短,与其他非人灵长类动物相比,越来越被认为是生物医学研究的重要非人灵长类动物(NHPs)。要最大限度地发挥圈养研究狨猴(包括基因操作动物)的作用,就必须使用辅助生殖技术(ART),包括操作、储存和共享狨猴精子。在此,我们确定了高质量精液样本的特征,并验证了一种选择高质量精子的简单方法:方法:使用计算机辅助精子分析(CASA)评估从44只狨猴采集的精液样本中精子的质量,并在半数样本中测试使用游动法挑选优质精子,以此作为优化体外受精或宫腔内人工授精的潜在方法:对于每个参考参数,低于或等于第5百分位数的样本被归类为异常精子,而高于第5百分位数的样本则被视为正常精子。在正常样本中,达到或超过第 50 百分位数的样本被归类为优质样本。优质精液样本具有以下特征:精液量≥30 µL;精子数量≥107/射精;总活力≥35%;正常形态≥5%。通过游动法分离的精子表现出更高的精子渐进运动能力(19.7% ± 4.5 vs. 5.6% ± 2.1; P = 0.01)和正常形态(13.1 ± 1.59 vs. 7.65 ± 1.1; P 结论:这项研究为评估狨猴精液样本确定了可靠的、有统计学支持的参考值,有助于确定最佳捐精者和选择用于辅助生殖的高质量精子样本。最终,这些参考值与经过验证的选择方法相结合,将有助于为国际共享基因多样化和/或基因编辑的狨猴精子用于研究和繁殖制定统一的标准。
Benchmarks defining high-quality sperm in the common marmoset (Callithrix jacchus).
Background: Common marmosets (Callithrix jacchus) are increasingly recognized as valuable nonhuman primates (NHPs) for biomedical research due to their small size and short reproductive cycle and lifespan relative to other NHP species. Maximizing the utility of captive research marmosets, including genetically manipulated animals, will require the use of assisted reproductive techniques (ART) including manipulation, storage, and sharing of marmoset sperm. Here, we identify characteristics of high-quality semen samples and validate a simple method for selecting high-quality sperm.
Methods: Computer-assisted sperm analysis (CASA) was used to evaluate sperm quality in semen samples collected from 44 marmosets and assessed the use of the swim-up method for the selection of high-quality sperm was also tested in half the samples as a potential means to optimize in vitro fertilization or intrauterine insemination.
Results: For each reference parameter, samples at or below the 5th percentile were categorized as abnormal sperm, while those above the 5th percentile were considered to be normal. Among normal samples, those at or above the 50th percentile were categorized as high-quality. High-quality semen samples exhibited the following characteristics: semen volume ≥ 30 µL; sperm count ≥ 107/ejaculate; total motility ≥ 35%; and normal morphology ≥ 5%. Sperm isolated by swim-up exhibited superior sperm progressive motility (19.7% ± 4.5 vs. 5.6% ± 2.1; P = 0.01) and normal morphology (13.1 ± 1.59 vs. 7.65 ± 1.1; P < 0.001) compared with unselected sperm.
Conclusion: This study defines robust, statistically supported reference values for evaluating marmoset semen samples to assist with the identification of optimal sperm donors and the selection of high-quality sperm samples for assisted reproduction. Ultimately, these reference values combined with a validated selection method will contribute to consistent standards for the international sharing of genetically diverse and/or gene-edited marmoset sperm for research and reproduction.
期刊介绍:
Andrology is the study of the male reproductive system and other male gender related health issues. Andrology deals with basic and clinical aspects of the male reproductive system (gonads, endocrine and accessory organs) in all species, including the diagnosis and treatment of medical problems associated with sexual development, infertility, sexual dysfunction, sex hormone action and other urological problems. In medicine, Andrology as a specialty is a recent development, as it had previously been considered a subspecialty of urology or endocrinology