{"title":"电针通过改善视网膜线粒体功能减缓实验性近视的发展：基于单细胞 RNA 测序的研究。","authors":"Jiawen Hao, Yunxiao Xie, Huixia Wei, Zhaohui Yang, Ruixue Zhang, Zhongyu Ma, Miao Zhang, Xiaoshi Du, Xuewei Yin, Jinpeng Liu, Bo Bao, Hongsheng Bi, Dadong Guo","doi":"10.1002/adbi.202400269","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to establish a complete atlas of retinal cells in lens-induced myopia (LIM) and electroacupuncture (EA) intervention by single-cell RNA sequencing (scRNA-seq) and to explore the potential mechanism of EA in improving experimental myopia progression in guinea pigs. scRNA-seq is used to assess changes in individual cellular gene levels in the retina of LIM- and EA-treated guinea pigs. In addition, the role of EA in slowing myopia progression by improving retinal mitochondrial function is further investigated. scRNA-seq identified ten cell clusters in the retina of LIM and EA guinea pigs and mitochondrial respiratory chain-related genes in Cones and Muller-glia cells-Cytochrome oxidase subunit III (COX3), NADH dehydrogenase subunit 4 (ND4), and NADH dehydrogenase subunit 2 (ND2) are closely related to lens-induced myopia. A comprehensive atlas in the retina of LIM and EA guinea pigs at a single-cell level is established, and the positive role of EA in improving retinal mitochondrial function to slow the experimental myopia progression in guinea pigs is revealed.</p>","PeriodicalId":7234,"journal":{"name":"Advanced biology","volume":" ","pages":"e2400269"},"PeriodicalIF":3.2000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Electroacupuncture Slows Experimental Myopia Progression by Improving Retinal Mitochondrial Function: A Study Based on Single-Cell RNA Sequencing.\",\"authors\":\"Jiawen Hao, Yunxiao Xie, Huixia Wei, Zhaohui Yang, Ruixue Zhang, Zhongyu Ma, Miao Zhang, Xiaoshi Du, Xuewei Yin, Jinpeng Liu, Bo Bao, Hongsheng Bi, Dadong Guo\",\"doi\":\"10.1002/adbi.202400269\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aimed to establish a complete atlas of retinal cells in lens-induced myopia (LIM) and electroacupuncture (EA) intervention by single-cell RNA sequencing (scRNA-seq) and to explore the potential mechanism of EA in improving experimental myopia progression in guinea pigs. scRNA-seq is used to assess changes in individual cellular gene levels in the retina of LIM- and EA-treated guinea pigs. In addition, the role of EA in slowing myopia progression by improving retinal mitochondrial function is further investigated. scRNA-seq identified ten cell clusters in the retina of LIM and EA guinea pigs and mitochondrial respiratory chain-related genes in Cones and Muller-glia cells-Cytochrome oxidase subunit III (COX3), NADH dehydrogenase subunit 4 (ND4), and NADH dehydrogenase subunit 2 (ND2) are closely related to lens-induced myopia. A comprehensive atlas in the retina of LIM and EA guinea pigs at a single-cell level is established, and the positive role of EA in improving retinal mitochondrial function to slow the experimental myopia progression in guinea pigs is revealed.</p>\",\"PeriodicalId\":7234,\"journal\":{\"name\":\"Advanced biology\",\"volume\":\" \",\"pages\":\"e2400269\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advanced biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1002/adbi.202400269\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/10/15 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1002/adbi.202400269","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/15 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0
摘要
本研究旨在通过单细胞RNA测序（scRNA-seq）建立透镜诱导近视（LIM）和电针（EA）干预下视网膜细胞的完整图谱，并探索EA改善豚鼠实验性近视进展的潜在机制。 scRNA-seq用于评估LIM和EA治疗豚鼠视网膜中单个细胞基因水平的变化。此外，还进一步研究了 EA 通过改善视网膜线粒体功能来减缓近视发展的作用。scRNA-seq在LIM和EA豚鼠视网膜中发现了十个细胞集群，在Cones和Muller-glia细胞中发现了线粒体呼吸链相关基因--细胞色素氧化酶亚基III（COX3）、NADH脱氢酶亚基4（ND4）和NADH脱氢酶亚基2（ND2）与透镜诱导的近视密切相关。在单细胞水平上建立了 LIM 和 EA 豚鼠视网膜的综合图谱，揭示了 EA 在改善视网膜线粒体功能以减缓豚鼠实验性近视进展方面的积极作用。
Electroacupuncture Slows Experimental Myopia Progression by Improving Retinal Mitochondrial Function: A Study Based on Single-Cell RNA Sequencing.
This study aimed to establish a complete atlas of retinal cells in lens-induced myopia (LIM) and electroacupuncture (EA) intervention by single-cell RNA sequencing (scRNA-seq) and to explore the potential mechanism of EA in improving experimental myopia progression in guinea pigs. scRNA-seq is used to assess changes in individual cellular gene levels in the retina of LIM- and EA-treated guinea pigs. In addition, the role of EA in slowing myopia progression by improving retinal mitochondrial function is further investigated. scRNA-seq identified ten cell clusters in the retina of LIM and EA guinea pigs and mitochondrial respiratory chain-related genes in Cones and Muller-glia cells-Cytochrome oxidase subunit III (COX3), NADH dehydrogenase subunit 4 (ND4), and NADH dehydrogenase subunit 2 (ND2) are closely related to lens-induced myopia. A comprehensive atlas in the retina of LIM and EA guinea pigs at a single-cell level is established, and the positive role of EA in improving retinal mitochondrial function to slow the experimental myopia progression in guinea pigs is revealed.
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