寡核苷酸乙酰化试剂对 RNA 的 2'-OH 进行位点特异性乙酰化修饰

IF 4.3 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Communications Pub Date : 2024-10-22 DOI:10.1039/d4cc04672b

Hirotaka Murase, Mio Eto, Jeongsu Lee, Yosuke Taniguchi, Shuhei Imoto, Shigeki Sasaki

{"title":"寡核苷酸乙酰化试剂对 RNA 的 2'-OH 进行位点特异性乙酰化修饰","authors":"Hirotaka Murase, Mio Eto, Jeongsu Lee, Yosuke Taniguchi, Shuhei Imoto, Shigeki Sasaki","doi":"10.1039/d4cc04672b","DOIUrl":null,"url":null,"abstract":"The method for a site-specific RNA acetylation using a novel oligonucleotide acetylating probe (Ac-probe) has been developed. It was confirmed that the acetyl group was site-specifically introduced to a 2'-OH of the sugar part of the target RNA via the formation of the RNA-probe duplex.","PeriodicalId":67,"journal":{"name":"Chemical Communications","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Site-specific acetyl modification of 2’-OH of RNA by oligonucleotide acetylating reagent\",\"authors\":\"Hirotaka Murase, Mio Eto, Jeongsu Lee, Yosuke Taniguchi, Shuhei Imoto, Shigeki Sasaki\",\"doi\":\"10.1039/d4cc04672b\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The method for a site-specific RNA acetylation using a novel oligonucleotide acetylating probe (Ac-probe) has been developed. It was confirmed that the acetyl group was site-specifically introduced to a 2'-OH of the sugar part of the target RNA via the formation of the RNA-probe duplex.\",\"PeriodicalId\":67,\"journal\":{\"name\":\"Chemical Communications\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-10-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical Communications\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1039/d4cc04672b\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Communications","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d4cc04672b","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

摘要

利用新型寡核苷酸乙酰化探针（Ac-probe）进行位点特异性 RNA 乙酰化的方法已经开发出来。研究证实，乙酰基是通过形成 RNA-探针双链体而特异性地引入到目标 RNA 糖部分的 2'-OH 位点上的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Site-specific acetyl modification of 2’-OH of RNA by oligonucleotide acetylating reagent

The method for a site-specific RNA acetylation using a novel oligonucleotide acetylating probe (Ac-probe) has been developed. It was confirmed that the acetyl group was site-specifically introduced to a 2'-OH of the sugar part of the target RNA via the formation of the RNA-probe duplex.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Chemical Communications 化学-化学综合

CiteScore

8.60

自引率

4.10%

发文量

2705

审稿时长

1.4 months

期刊介绍： ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.