Ehab A. D. Sarhan, Salama A. S. El-Blasy, Ahmed A. Kheder
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引用次数: 0
摘要
2023 年 2 月，在埃及基纳省 Qus 市 Hegazah 村（25°50'49 "N 32°49'33 "E）的一块商品田里进行例行田间调查时，发现当地种植的葫芦巴（Trigonella foenum-graecum L.）叶片上出现了白粉病症状。显微特征显示，分生孢子梗圆柱形，透明，直，直立到弯曲，单个形成分生孢子，初级分生孢子顶部狭窄，披针形，钝到尖形，次级分生孢子椭圆形或圆柱形，量度。芽管靠近基部。BLASTn 结果表明，其 ITS rDNA 与提交的 Leveillula taurica 序列具有 100% 的相似性，通过最大似然法获得的系统发生树证实了 BLASTn 的结果。形态学和分子方法证实了 Leveillula taurica (Lév.) G. Arnaud 的身份。获得的 ITS rDNA 序列已存入 NCBI GenBank（OR472546）。对该真菌进行了致病性试验，结果符合科赫假说。据我们所知，这是埃及首次报道由 L. taurica (Lév.) G. Arnaud.引起的葫芦巴白粉病。
First report of Leveillula Taurica causing powdery mildew on Fenugreek in Egypt
During routine field surveys in February 2023, in a commercial field located in Hegazah village, Qus, Qena governorate, Egypt (25°50’49"N 32°49’33"E), powdery mildew symptoms were observed on leaves of a local cultivar of fenugreek (Trigonella foenum-graecum L.). Microscopic characterization showed that conidiophores were cylindrical, hyaline, straight, erect to bent, forming conidia singly, primary conidia were apically narrowed, lanceolate, obtuse to pointed measuring, secondary conidia were ellipsoid or cylindrical, measuring. Germ tubes were close to the base. BLASTn results showed 100% similarity with the submitted sequences of Leveillula taurica for ITS rDNA, the obtained phylogenetic tree through the maximum likelihood method confirmed the BLASTn results. Morphological and molecular approaches confirmed the identity of Leveillula taurica (Lév.) G. Arnaud. The obtained ITS rDNA sequence was deposited in NCBI GenBank (OR472546). Pathogenicity tests were conducted, and Koch’s postulates were fulfilled with the fungus. To our knowledge, this is the first report of powdery mildew caused by L. taurica (Lév.) G. Arnaud. on fenugreek in Egypt.
期刊介绍：
Australasian Plant Disease Notes is an international journal for publication of short reports from all countries which describe original research that transcends national boundaries. All aspects of plant pathology are covered including new geographical records of diseases or pathogens, new pathogens or pathogen strains, taxonomic papers, quarantine notes, disease management and diagnostic methods. Australasian Plant Disease Notes is a publication of the Australasian Plant Pathology Society.
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