一种体外测定激素或激动剂刺激的脂肪酶活性的方法

Prostaglandins, leukotrienes, and medicine Pub Date : 1987-06-01 DOI:10.1016/0262-1746(87)90046-1

Donald J. Fretland, Peter S. Cammarata

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引用次数: 0

摘要

一种在体外测量激素或激动剂刺激的脂肪酶活性的实验，使用外源性前列腺素合成酶作为释放花生四烯酸的陷阱。先前在体内测试的26种激素和激动剂中的15种，在体外进行了测试，刺激或抑制花生四烯酸的释放与其在体内的活性平行。加压素刺激花生四烯酸在10−11 M至10−5 M范围内呈- 5线性、对数剂量释放。

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An in vitro method for assay of hormone or agonist-stimulated lipase activity

An assay for $in vitro$ measurement of hormone or agonist-stimulated lipase activity, using exogenous prostaglandin synthetase as a trap for liberated arachidonic acid is described. Fifteen of twenty-six hormones and agonists previously tested $in vivo$ , were tested $in vitro$ and either stimulated or inhibited release of arachidonic acid parallel to their activity $in vivo$ . Vasopressin stimulated the release of arachidonic acid in ⁻⁵ linear, log-dose manner, from 10⁻¹¹ M to 10⁻⁵ M.

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Prostaglandins, leukotrienes, and medicine

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