以葫芦[7]脲-硫苯基吡啶鎓复合物为宿主-染料报告物对进行荧光ON置换测定

IF 4.9 1区化学 Q1 CHEMISTRY, ORGANIC

Organic Letters Pub Date : 2024-10-14 DOI:10.1021/acs.orglett.4c03469

Mohammad A. Alnajjar, Andreas Hennig

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引用次数: 0

摘要

N- 甲基-4-噻吩吡啶阳离子（ThioPy）是葫芦[7]脲（CB7）的一种高亲和力（Kd 约为 5 nM）、快速交换荧光探针。在传感应用中，CB7/ThioPy 复合物在被分析物置换后会显示出独特的荧光开启响应。此外，CB7/ThioPy 的脂质体封装在大单酰胺囊（LUVs）中，为了解囊内染料位移反应提供了机理。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Fluorescence Turn-ON Displacement Assays with Cucurbit[7]uril–Thiophenylpyridinium Complexes as Host–Dye Reporter Pairs

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Fluorescence Turn-ON Displacement Assays with Cucurbit[7]uril–Thiophenylpyridinium Complexes as Host–Dye Reporter Pairs

The N-methyl-4-thiophenylpyridinium cation (ThioPy) is a high affinity (K_d ca. 5 nM), fast-exchanging fluorescent probe for cucurbit[7]uril (CB7). The CB7/ThioPy complex shows a unique fluorescence turn-ON response upon displacement by an analyte in sensing application. This enabled the development of a real-time fluorescence assay with the MRFA peptide for the protease thermolysin, which is also suitable for the cancer biomarker cathepsin B. Moreover, liposome encapsulation of CB7/ThioPy in large unilamellar vesicles (LUVs) provided mechanistic insight into intravesicular dye displacement reactions.

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来源期刊

Organic Letters 化学-有机化学

CiteScore

9.30

自引率

11.50%

发文量

1607

审稿时长

1.5 months

期刊介绍： Organic Letters invites original reports of fundamental research in all branches of the theory and practice of organic, physical organic, organometallic,medicinal, and bioorganic chemistry. Organic Letters provides rapid disclosure of the key elements of significant studies that are of interest to a large portion of the organic community. In selecting manuscripts for publication, the Editors place emphasis on the originality, quality and wide interest of the work. Authors should provide enough background information to place the new disclosure in context and to justify the rapid publication format. Back-to-back Letters will be considered. Full details should be reserved for an Article, which should appear in due course.