组蛋白甲基转移酶 WHSC1 在浸润性乳腺癌中的表达及其与临床和病理数据的相关性

IF 2.9 4区医学 Q2 PATHOLOGY

Pathology, research and practice Pub Date : 2024-10-06 DOI:10.1016/j.prp.2024.155647

Wei Lin , Xian Wu , Sunwang Xu , Dexing Wang , Jinshu Chen , Linying Chen , Xiangjin Chen

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引用次数: 0

摘要

背景WHSC1蛋白可促进组蛋白H3在K36位置的特异性二甲基化，从而增强基因转录和表达。研究证实它在各种恶性肿瘤中的高表达。我们的目的是找出新的分子标记物来评估乳腺癌细胞的生物学行为。方法我们根据 TCGA 数据对乳腺癌和邻近组织的 mRNA 表达进行了全面分析。我们纳入了 2012 年至 2016 年期间在福建医科大学附属第一医院接受治疗的 141 例乳腺癌患者。我们获得了患者的临床信息和病理标本。我们采用了组织芯片（TMA）技术。我们采用卡方检验进行组间比较，以 p < 0.05 表示统计学意义。此外，我们还分析了WHSC1表达与临床或病理数据之间的关联。结果WHSC1 mRNA在乳腺癌组织中的表达明显高于邻近组织（p < 0.001）。此外，乳腺癌中 WHSC1 蛋白的高表达与几个重要的临床参数有关，如病理类型（p = 0.007）、高 Ki67 表达（Ki67＞20 %）（p < 0.001）、淋巴结转移（p <0.001）、T期（p = 0.011）、N期（p <0.001）、术后病理分期（p <0.001）、绝经前状态（p = 0.004）和HER2阳性状态（p <0.001）。多变量回归分析表明，WHSC1高表达、Ki67水平升高和HER2阳性是乳腺癌患者发生腋窝淋巴结转移的独立危险因素。

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Expression of histone methyltransferase WHSC1 in invasive breast cancer and its correlation with clinical and pathological data

Background

The WHSC1 protein facilitates specific dimethylation of histone H3 at the K36 position, enhancing gene transcription and expression. Studies have confirmed its high expression in diverse malignant tumors. We aimed to identify novel molecular markers to assess the biological behavior of breast cancer cells.

Methods

We conducted a comprehensive analysis of mRNA expression in breast cancer and adjacent tissues based on TCGA data. We enrolled 141 breast cancer patients treated at the First Affiliated Hospital of Fujian Medical University between 2012 and 2016. Patient clinical information and pathological specimens were obtained. We utilized tissue microarray (TMA) technology. We employed the chi-square test for between-group comparisons, with p < 0.05 indicating statistical significance. Furthermore, we analyzed the associations between WHSC1 expression and clinical or pathological data.

Results

WHSC1 mRNA expression was significantly higher in breast cancer tissues than in adjacent tissues (p < 0.001). Moreover, high WHSC1 protein expression in breast cancer was associated with several important clinical parameters, such as pathological type (p = 0.007), high Ki67 expression(Ki67＞20 %) (p < 0.001), lymph node metastasis (p < 0.001), T stage (p = 0.011), N stage (p < 0.001), postoperative pathological stage (p < 0.001), premenopausal status (p = 0.004), and positive HER2 status (p < 0.001). Multivariate regression analysis showed that high WHSC1 expression, elevated Ki67 levels, and positive HER2 status were independent risk factors for axillary lymph node metastasis in breast cancer patients.

Conclusion

WHSC1 protein expression is upregulated in breast cancer patients and represents an independent risk factor influencing axillary lymph node metastasis, highlighting its potential significance as a strong candidate biomarker.

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来源期刊

Pathology, research and practice 医学-病理学

CiteScore

5.00

自引率

3.60%

发文量

405

审稿时长

24 days

期刊介绍： Pathology, Research and Practice provides accessible coverage of the most recent developments across the entire field of pathology: Reviews focus on recent progress in pathology, while Comments look at interesting current problems and at hypotheses for future developments in pathology. Original Papers present novel findings on all aspects of general, anatomic and molecular pathology. Rapid Communications inform readers on preliminary findings that may be relevant for further studies and need to be communicated quickly. Teaching Cases look at new aspects or special diagnostic problems of diseases and at case reports relevant for the pathologist''s practice.