Sabrina D. Zeppa , Matteo Micucci , Fabio Ferrini , Anna M. Gioacchini , Giovanni Piccoli , Lucia Potenza , Alessia Bartolacci , Giosuè Annibalini , Asher A. Rehman , Cinzia Calcabrini , Vilberto Stocchi , Piero Sestili
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This research endeavour is designed to scrutinise the phytochemical composition and cytotoxic effects of an alcoholic extract derived from the pericarps of <em>G. mangostana</em> (ME) on leukaemic cell lines.</div></div><div><h3>Methods</h3><div><em>G. mangostana</em> L. pericarps were subjected to ethanol extraction, and subsequent compound identification was conducted via Capillary Liquid Chromatography-Electrospray Ionisation-Quadrupole Time-of-Flight Tandem Mass Spectrometry (CapLC-ESI-QTOF-MS/MS). In parallel, the extract was fractionated through High-Performance Liquid Chromatography (HPLC). Furthermore, U937, Jurkat and normal human umbilical vein endothelial cell (HUVEC) lines were exposed to varying concentrations of the extract to assess cell proliferation, viability, cytotoxicity, and apoptotic DNA damage.</div></div><div><h3>Results</h3><div>Investigations confirmed the presence of various xanthones, including β-mangostin, α-mangostin, and garcinone E. Significantly, ME displayed pronounced cytotoxic effects specifically on leukaemic cells, distinguishing its impact on malignant cells as opposed to non-malignant ones, and facilitated apoptotic DNA fragmentation. Moreover, the whole extract consistently displayed greater cytotoxicity compared to individual fractions.</div></div><div><h3>Conclusion</h3><div>According to the results obtained ME selective cytotoxicity against cancer cell lines and greater biological activity than the single compounds in the extract. 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引用次数: 0
摘要
引言 藤黄属植物(Garcinia mangostana L.)因其传统的药用价值和在营养保健领域日益增长的相关性而得到广泛认可。本研究旨在仔细研究从芒果树果皮(ME)中提取的酒精提取物的植物化学成分和对白血病细胞系的细胞毒性作用。方法对芒果树果皮进行乙醇提取,然后通过毛细管液相色谱-电喷雾离子化-四极杆飞行时间串联质谱法(CapLC-ESI-QTOF-MS/MS)进行化合物鉴定。同时,提取物还通过高效液相色谱法(HPLC)进行了分馏。此外,将 U937、Jurkat 和正常人脐静脉内皮细胞 (HUVEC) 株暴露于不同浓度的提取物中,以评估细胞增殖、活力、细胞毒性和凋亡 DNA 损伤。值得注意的是,ME 对白血病细胞有明显的细胞毒性作用,对恶性细胞的影响有别于对非恶性细胞的影响,并能促进 DNA 的凋亡破碎。此外,与单个馏分相比,整个提取物始终显示出更强的细胞毒性。这些发现凸显了 ME 在预防癌症方面的潜力,在药物干预之前对饮食策略进行了补充。
Differential cytotoxic effects of Garcinia mangostana pericarp extract on leukaemic versus normal human cell lines: insights into selective anticancer activity
Introduction
Garcinia mangostana L. is widely recognised for its traditional medicinal uses and growing relevance in the nutraceutical sector. This research endeavour is designed to scrutinise the phytochemical composition and cytotoxic effects of an alcoholic extract derived from the pericarps of G. mangostana (ME) on leukaemic cell lines.
Methods
G. mangostana L. pericarps were subjected to ethanol extraction, and subsequent compound identification was conducted via Capillary Liquid Chromatography-Electrospray Ionisation-Quadrupole Time-of-Flight Tandem Mass Spectrometry (CapLC-ESI-QTOF-MS/MS). In parallel, the extract was fractionated through High-Performance Liquid Chromatography (HPLC). Furthermore, U937, Jurkat and normal human umbilical vein endothelial cell (HUVEC) lines were exposed to varying concentrations of the extract to assess cell proliferation, viability, cytotoxicity, and apoptotic DNA damage.
Results
Investigations confirmed the presence of various xanthones, including β-mangostin, α-mangostin, and garcinone E. Significantly, ME displayed pronounced cytotoxic effects specifically on leukaemic cells, distinguishing its impact on malignant cells as opposed to non-malignant ones, and facilitated apoptotic DNA fragmentation. Moreover, the whole extract consistently displayed greater cytotoxicity compared to individual fractions.
Conclusion
According to the results obtained ME selective cytotoxicity against cancer cell lines and greater biological activity than the single compounds in the extract. These findings underscore ME's potential in cancer prevention, complementing dietary strategies ahead of pharmaceutical interventions.
期刊介绍:
The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.