质谱法证明代表性不足的 RNA 蛋白交叉连接

IF 4.3 3区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

ACS Omega Pub Date : 2024-09-25 DOI:10.1021/acsomega.4c0643610.1021/acsomega.4c06436

Jong Woo Bae*,

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引用次数: 0

摘要

在蛋白质-RNA 复合物中，紫外线主要使蛋白质与尿嘧啶交联，而使其他核碱基交联的程度较小。交联的 RNA 分子可通过氢氟化物消化进行简化，并通过串联质谱进行鉴定。虽然尿苷或尿嘧啶交联已成功定性，但还缺乏其他 UVC 交联 RNA 碱基的具体证据。在此，我阐明了紫外线交联、氢氟化物消化的鸟嘌呤、胞苷和甲基腺嘌呤的质谱特征。这些信息有助于我们更详细地捕捉原生蛋白质与 RNA 的相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Mass Spectrometric Evidence for the Under-represented RNA–Protein Cross-Links

In protein–RNA complexes, UVC cross-links proteins predominantly to uracil and other nucleobases to a smaller extent. The cross-linked RNA moieties can be simplified via hydrofluoride digestion and identified via tandem mass spectrometry. While uridine or uracil cross-links have been successfully characterized, concrete evidence for other UVC-cross-linked RNA bases is lacking. Here, I elucidate mass spectrometric signatures of UVC-cross-linked, hydrofluoride-digested guanine, cytidine, and methyladenine. This information can help us capture the native protein–RNA interactions in more detail.

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来源期刊

ACS Omega Chemical Engineering-General Chemical Engineering

CiteScore

6.60

自引率

4.90%

发文量

3945

审稿时长

2.4 months

期刊介绍： ACS Omega is an open-access global publication for scientific articles that describe new findings in chemistry and interfacing areas of science, without any perceived evaluation of immediate impact.