马冠状病毒在马肠道内的感染和复制。

IF 3.7 1区农林科学 Q1 VETERINARY SCIENCES

Veterinary Research Pub Date : 2024-10-10 DOI:10.1186/s13567-024-01381-z

Yoshinori Kambayashi, Manabu Nemoto, Akihiro Ochi, Daiki Kishi, Takanori Ueno, Koji Tsujimura, Hiroshi Bannai, Nanako Kawanishi, Minoru Ohta, Tohru Suzuki

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引用次数: 0

摘要

本研究从十二指肠、空肠和回肠中提取了马肠肠杆菌（EIEs），并接种了马冠状病毒（ECoV），以研究它们是否适合作为研究ECoV感染的体外模型。免疫组化结果显示，EIEs由肠上皮细胞在体内表达的各种细胞类型组成。定量反转录聚合酶链反应（qRT-PCR）和病毒滴定显示，ECoV感染了EIEs并在其中复制。电子显微镜也证实了这些结果。这项研究表明，EIEs可作为研究马肠道上皮细胞与ECoV相互作用的新型体外工具。

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Equine coronavirus infection and replication in equine intestinal enteroids.

In this study, equine intestinal enteroids (EIEs) were generated from the duodenum, jejunum, and ileum and inoculated with equine coronavirus (ECoV) to investigate their suitability as in vitro models with which to study ECoV infection. Immunohistochemistry revealed that the EIEs were composed of various cell types expressed in vivo in the intestinal epithelium. Quantitative reverse-transcription PCR (qRT-PCR) and virus titration showed that ECoV had infected and replicated in the EIEs. These results were corroborated by electron microscopy. This study suggests that EIEs can be novel in vitro tools for studying the interaction between equine intestinal epithelium and ECoV.

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来源期刊

Veterinary Research 农林科学-兽医学

CiteScore

7.00

自引率

4.50%

发文量

审稿时长

3 months

期刊介绍： Veterinary Research is an open access journal that publishes high quality and novel research and review articles focusing on all aspects of infectious diseases and host-pathogen interaction in animals.