植物乳杆菌 P101 通过 AMPK 信号通路缓解酒精诱导的小鼠肝脂质积累:肠道微生物群和代谢组学分析。

IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Xiaoyan Feng, Mengqi Wang, Siyue Wen, Liehai Hu, Yuzhi Lan, Hengyi Xu
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引用次数: 0

摘要

减轻脂肪变性对延缓酒精性肝病的进展至关重要。我们的研究探讨了植物乳杆菌 P101(LP.P101)对缓解酒精诱导的肝脏脂质蓄积的作用和机制。小鼠模型通过短期(10天)加暴饮暴食乙醇喂养建立,每天灌胃108 CFU/mL的LP.P101。LP.101 对 AMPK 激活的干预显著减少了肝脏中的脂滴。然而,当多索吗啡抑制AMPK时,肝脏中的相关指标(ALT、TG等)和AMPK及相关基因的表达水平与酒精喂养组趋同。与酒精喂养组相比,LP.P101降低了固缩菌的相对丰度,增加了类杆菌的相对丰度。副乳杆菌(Parabacteroides merdae)与脂质积累呈负相关,未分类的负杆菌(Negativibacillus)与AMPK激活呈负相关。重要的是,LP.P101 改变了血清代谢物的组成。潜在的生物标志物stercobilinogen与AMPK活化呈正相关,与脂质积累呈负相关。这项研究证实,LP.P101 通过激活 AMPK 可减轻酒精诱导的小鼠肝脏脂质积累,而肠道微生物群和代谢物的改变可能在激活 AMPK 方面发挥了重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Lactiplantibacillus plantarum P101 Alleviated Alcohol-Induced Hepatic Lipid Accumulation in Mice via AMPK Signaling Pathway: Gut Microbiota and Metabolomics Analysis.

Mitigating steatosis is essential for delaying the progression of alcoholic liver disease. The effect and mechanism of Lactiplantibacillus plantarum P101 (LP.P101) on alleviating alcohol-induced hepatic lipid accumulation were investigated in our study. The mouse model was constructed by a short-term (10-day)-plus-binge ethanol feeding and gavaged with 108 CFU/mL of LP.P101 daily. Lipid droplet in the liver was significantly reduced by LP.101 intervention on AMPK activation. However, when AMPK was inhibited by dorsomorphin, the levels of related indicators (ALT, TG, etc.) and the expression levels of AMPK and relevant genes in the liver converged to that of the alcohol-fed group. Compared with the alcohol-fed group, LP.P101 reduced the relative abundance of Firmicutes and increased that of Bacteroidetes. Parabacteroides merdae was negatively correlated with lipid accumulation, and unclassified Negativibacillus was negatively associated with AMPK activation. Importantly, LP.P101 modified the compositions of the serum metabolites. The potential biomarker stercobilinogen was positively correlated with AMPK activation and negatively associated with lipid accumulation. This work confirmed that LP.P101 attenuated alcohol-induced hepatic lipid accumulation in mice through AMPK activation, and the alterations in gut microbiota and metabolites may play a significant role on AMPK activation.

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来源期刊
Probiotics and Antimicrobial Proteins
Probiotics and Antimicrobial Proteins BIOTECHNOLOGY & APPLIED MICROBIOLOGYMICROB-MICROBIOLOGY
CiteScore
11.30
自引率
6.10%
发文量
140
期刊介绍: Probiotics and Antimicrobial Proteins publishes reviews, original articles, letters and short notes and technical/methodological communications aimed at advancing fundamental knowledge and exploration of the applications of probiotics, natural antimicrobial proteins and their derivatives in biomedical, agricultural, veterinary, food, and cosmetic products. The Journal welcomes fundamental research articles and reports on applications of these microorganisms and substances, and encourages structural studies and studies that correlate the structure and functional properties of antimicrobial proteins.
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