由诱导剂介导的氯唑嗪和熊果酸在苹果皮衍生胼胝体中的同时积累。

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2024-10-10 DOI:10.1002/jsfa.13955

Carmen Laezza, Maria Maisto, Paola Imbimbo, Daria Maria Monti, Mariavittoria Verrillo, Antonio Di Loria, Simona Maria Monti, Adua Marzocchi, Paolo Grieco, Gian Carlo Tenore, Vincenzo D'Amelia, Maria Manuela Rigano

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引用次数: 0

摘要

背景：苹果皮含有丰富的天然分子，其中许多具有显著的生物活性。在本研究中，我们的目标是建立一个新的胼胝体系，该胼胝体系来源于意大利当地品种 Annurca 的苹果皮，众所周知，该品种积累了大量的二氢查耳酮和萜烯。为此，我们测试了一种诱导剂（酵母提取物）对参与叶绿素和熊果酸生物合成的关键酶编码基因表达的影响，从而导致这些抗氧化化合物的积累。我们还评估了富含这些植物化学物质的胼胝体提取物的生物活性：结果：诱导后的数据显示，与叶绿素和熊果酸合成直接相关的基因表达增加，这些基因在培养物中积累。这大概可以解释被激发的芹菜提取物在抑制金黄色葡萄球菌和蜡样芽孢杆菌生长方面的显著活性。此外，富含抗氧化化合物的提取物还能抑制暴露在紫外线-A（UV-A）辐射下的人体细胞中活性氧（ROS）的产生：我们的研究结果凸显了苹果皮细胞系在生产天然化合物方面的巨大潜力，这些天然化合物可用作促进人类健康的食品成分。© 2024 The Author(s).John Wiley & Sons Ltd 代表化学工业协会出版的《食品与农业科学杂志》。

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Elicitor-mediated simultaneous accumulation of phloridzin and ursolic acid in Annurca apple peel-derived calli.

Background: Apple peel is rich in natural molecules, many exhibiting a significant bioactivity. In this study, our objective was to establish a novel callus line derived from the apple peel of the Italian local variety Annurca, known to accumulate high levels of dihydrochalcones and terpenes. In this regard, we tested the impact of one elicitor, yeast extract, on the expression of genes encoding key enzymes involved in phloridzin and ursolic acid biosynthesis, leading to the accumulation of these antioxidant compounds. We also assessed the bioactivity of callus extracts enriched in these phytochemicals.

Results: After the elicitation, data showed increased expression of genes directly related to the synthesis of phloridzin and ursolic acid that were found to accumulate within the cultures. This presumably could explain the remarkable activity of extracts from the elicited-calli in inhibiting the growth of Staphylococcus aureus and Bacillus cereus. Also, the extracts enriched in antioxidant compounds inhibited reactive oxygen species (ROS) production in human cells exposed to ultraviolet-A (UV-A) radiation.

Conclusion: Our results underscore the vast potential of the Annurca apple peel cell line in producing natural compounds that can be employed as food components to promote human health. © 2024 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.