B Bernal, I Gallarosa-García, A Toledano-Díaz, C Castaño, R Velázquez, O Torres, M G Gil, J Santiago-Moreno
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引用次数: 0
摘要
1.本研究评估了不同抗氧化剂对红腿鹧鸪冷藏/冷冻解冻精子质量的影响。采集 40 只红腿鹧鸪的精子样本，用 Lake 和 Ravie 84 以 1:1 （v:v）的比例混合，并添加 0、0.2、0.4 和 0.8 mM 的抗坏血酸或丁基羟基甲苯（BHT），以及 0、100、200 和 300 IU/ml 的过氧化氢酶（CAT）或超氧化物歧化酶（SOD）。每个浓度使用 10 个精子样本。对新鲜精子、在 5°C 冷冻 6 小时后的精子或冷冻-解冻后的精子3 的运动能力和存活率进行评估。对于冷冻后的精子，抗坏血酸的存在降低了存活率和几个运动变量；BHT 0.8 mM 增加了非进行性运动（NPM，26.7 ± 1.99 vs. 20.7 ± 2.12）；CAT 200 IU/ml 提高了直线运动速度（40.4 ± 4.63 μ/s vs. 29.9 ± 4.62 μ/s）。29.9±4.62μ/s）和线性进展率（52.8±3.11% vs. 45.4±2.98%）；SOD 100 IU/ml 增加了 NPM（24.5±1.21% vs. 19.3±1.75%），并倾向于改善总进展运动（42.7±3.33% vs. 33.2±3.26%，p = 0.07）。使用添加了 CAT 200 或 SOD 100 的扩展剂并不能提高解冻后的精子质量。 本研究为优化红腿鹧鸪精子的冷藏和冷冻方案提供了新的思路。
Catalase, superoxide dismutase and butylated hydroxytoluene benefit mid-term storage of red-legged partridge sperm (Alectoris rufa).
1. The present study assessed the effect of different antioxidants on the quality of chilled/frozen-thawed sperm of red-legged partridge.2. Sperm samples from 40 red-legged partridges were collected and extended 1:1 (v:v) with Lake and Ravie 84, supplemented with ascorbic acid or butylated hydroxytoluene (BHT) at 0, 0.2, 0.4, 0.8 mM and catalase (CAT) or superoxide dismutase (SOD) at 0, 100, 200 and 300 IU/ml. Ten sperm samples were used per concentration. Motility and viability were evaluated in fresh and after 6 h of chilling at 5°C or after freezing-thawing.3. For chilled sperm, the presence of ascorbic acid decreased viability and several motility variables; BHT 0.8 mM increased non-progressive motility (NPM, 26.7 ± 1.99 vs. 20.7 ± 2.12); CAT 200 IU/ml improved the rectilinear velocity (40.4 ± 4.63 μ/s vs. 29.9 ± 4.62 μ/s) and linear progression ratio (52.8 ± 3.11% vs. 45.4 ± 2.98%); SOD 100 IU/ml increased NPM (24.5 ± 1.21% vs. 19.3 ± 1.75%) and tended to improve total progressive motility (42.7 ± 3.33% vs. 33.2 ± 3.26%, p = 0.07). Using an extender supplemented with CAT 200 or SOD 100 did not improve the post-thawed sperm quality.4. The present work provides an advance in the optimisation of chilling and freezing protocols for red-legged partridge sperm.
期刊介绍：
From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .
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