利用顺式二醇-微孔有机网络与 PT-SPE 和 HPLC-MS/MS 技术增强对人体尿液中儿茶酚胺的检测。

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A Pub Date : 2024-09-30 DOI:10.1016/j.chroma.2024.465408

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引用次数: 0

摘要

通过室温和 Sonogashira 偶联反应合成了一种新型顺式二元醇-微孔有机网络（MONs-2OH）材料，该材料对儿茶酚胺（CA）具有优异的吸附性能。MONs-2OH 显示出强大的氢键和 π-π 堆积相互作用，这对有效吸附至关重要。将 MONs-2OH 加入移液器吸头固相萃取中，开发出一种使用 HPLC-MS/MS 检测人体尿液中 CAs 的新方法。该吸附剂具有稳定性高、比表面积大、酚羟基丰富、萃取速度快、吸附效率高等特点。该方法线性范围宽（0.5-500 ng/mL），检出限低（0.06-0.26 ng/mL），准确度高（90.4%-99.4%），精密度好（RSD ≤ 10%）。比较研究表明，MONs-2OH 在回收率和吸附容量方面优于商用吸附剂。这些结果凸显了 MONs-2OH 在快速、灵敏测定 CAs 方面的潜力，为抑郁症的辅助诊断提供了显著优势，并提高了 PT-SPE 在样品预处理中的应用。

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Enhanced detection of catecholamines in human urine using Cis-diol-microporous organic networks with PT-SPE and HPLC-MS/MS

A novel cis-diol-microporous organic networks (MONs-2OH) material was synthesized via room temperature and Sonogashira coupling reactions, which exhibits exceptional adsorption properties for catecholamines (CAs). MONs-2OH demonstrates robust hydrogen bonding and π-π stacking interactions, crucial for effective adsorption. The MONs-2OH was incorporated into pipette tip solid-phase extraction and developed a new method for detecting CAs in human urine using HPLC-MS/MS. Characterization of the adsorbent revealed its high stability, large specific surface area, abundant phenolic hydroxyl groups, rapid extraction speed, and superior adsorption efficiency. The method achieved a wide linear range (0.5–500 ng/mL), low detection limits (0.06–0.26 ng/mL), high accuracy (90.4 %-99.4 %), and excellent precision (RSD ≤ 10 %). Comparative studies showed MONs-2OH outperforms commercial adsorbents in terms of recovery and adsorption capacity. The results underscore the potential of MONs-2OH for rapid and sensitive CAs determination, offering significant advantages for the auxiliary diagnosis of depression and enhancing the application of PT-SPE in sample pretreatment.

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来源期刊

Journal of Chromatography A 化学-分析化学

CiteScore

7.90

自引率

14.60%

发文量

742

审稿时长

45 days

期刊介绍： The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.