开发用于自主电子显微镜的 mK 温度控制无液氦低温样品支架。

IF 2.1 3区 工程技术 Q2 MICROSCOPY
X. He , R. Kostin , E. Knight , M.G. Han , J. Mun , I. Bozovic , C. Jing , Y. Zhu
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引用次数: 0

摘要

自动和自主低温透射电子显微镜(Cryo-EM)要求样品架能够保持 10 K 以下的温度,并且能够精确控制、保持时间长、氦气用量少。为了应对这一挑战,我们启动了一个雄心勃勃的项目,开发一种基于闭合循环低温冷却器的新型低温样品架,该样品架无需使用液氦和消耗气态氦。这篇文章介绍了最初原型的设计、建造和实验测试,它的极限温度达到了 5.6 K,稳定性接近 1mK,同时提供了从 295 K 到 5.6 K 的宽温度控制范围,标志着低温电子显微镜支架开发的明显进步。虽然原型并非为原子分辨率成像而设计,因此缺乏一个坚固的支撑系统来减轻低温冷却器脉冲管产生的机械振动,但这种创新方法成功地证明了概念。它为材料和生物科学领域最先进的低温显微镜和显微分析提供了前所未有的能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of a liquid-helium free cryogenic sample holder with mK temperature control for autonomous electron microscopy
The automated and autonomous cryogenic transmission electron microscopy (Cryo-EM) demands a sample holder capable of maintaining temperatures below 10 K with precise control, long holding times, and minimal helium use. Rising to this challenge, we initiated an ambitious project to develop a novel closed-cycle cryocooler-based cryogenic sample holder that operates without the use of liquid helium and the consumption of gaseous helium. This article presents the design, construction, and experimental testing of the initial prototype, which achieves an ultimate temperature of 5.6 K with exceptional stability close to 1mK, while providing a wide temperature control range from 295 K to 5.6 K, marking a clear advancement in cryo-EM holder development. While the prototype was not designed for atomic resolution imaging and thus lacks a sturdy support system to mitigate mechanical vibrations from the cryocooler's pulsed tube, this innovative approach successfully demonstrates proof of concept. It offers unprecedented capabilities for state-of-the-art cryogenic microscopy and microanalysis in materials and biological sciences.
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来源期刊
Ultramicroscopy
Ultramicroscopy 工程技术-显微镜技术
CiteScore
4.60
自引率
13.60%
发文量
117
审稿时长
5.3 months
期刊介绍: Ultramicroscopy is an established journal that provides a forum for the publication of original research papers, invited reviews and rapid communications. The scope of Ultramicroscopy is to describe advances in instrumentation, methods and theory related to all modes of microscopical imaging, diffraction and spectroscopy in the life and physical sciences.
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