{"title":"Aporrectodea caliginosa蚯蚓暴露于单一和混合杀虫剂处理(Prosaro 和 Decis)后的应激生物标志物反应。","authors":"Djamila Hafsi, Ibtissem Sbartai, Hana Sbartai","doi":"10.1007/s10646-024-02811-6","DOIUrl":null,"url":null,"abstract":"<p><p>This study aims to assess the impact of two pesticides commonly used in Algeria (Prosaro XRT and Decis 25 EC), as well as their combinations at recommended doses, on a non-target species bioindicator of soil pollution, the earthworm Aporrectodea caliginosa, using physiological (mortality and growth) and biochemical parameters (proteins, glutathione, catalase activity and glutathione S-transferase, acetylcholine esterase, lipoxygenase). The recommended dose and its double were tested individually and in combination for this. It should be noted that the protocol used and the initial concentrations selected are the same as those used in the field. After 7 and 14 days (7D/14D) of exposure, all dosages were administered. Our findings show that the pesticides tested had no effect on earthworm survival. However, a significant decrease in their growth rates depending on the different concentrations was observed for the different treatments over the entire exposure period of 7 or 14 D. The greatest reductions (31.62%, 35.04%) are reported after 14D for the high concentrations of Decis alone (D2) as well as for the combined treatment Prosaro/Decis (P2/D2). At the same time, an increase in total protein contents (more than 50% after 14D) as well as a decrease in acetylcholine esterase activity were reported for all treatments. We were also able to identify the induction of oxidative stress after xenobiotic exposure, which is more pronounced at the end of the treatment (14D), resulting in the stimulation of the antioxidant system (gluthione, glutathione S-transférase, catalase) as well as the induction of lipoxygenase, which is responsible for the oxidation of polyunsaturated fatty acids as well as the generation of reactive oxygen species (ROS) involved in the inflammatory phenomenon. Finally, it turns out that the species Aporrectodea caliginosa is sensitive to the different concentrations applied, even those used in the open field, and that Decis (deltamethrin) seems to be more toxic than Prosaro and that the combinaison P2/D2 is as toxic as Decis alone (D2).</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":"1180-1192"},"PeriodicalIF":2.4000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Stress biomarker response in Aporrectodea caliginosa earthworms exposed to single and combined pesticide treatments (Prosaro and Decis).\",\"authors\":\"Djamila Hafsi, Ibtissem Sbartai, Hana Sbartai\",\"doi\":\"10.1007/s10646-024-02811-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aims to assess the impact of two pesticides commonly used in Algeria (Prosaro XRT and Decis 25 EC), as well as their combinations at recommended doses, on a non-target species bioindicator of soil pollution, the earthworm Aporrectodea caliginosa, using physiological (mortality and growth) and biochemical parameters (proteins, glutathione, catalase activity and glutathione S-transferase, acetylcholine esterase, lipoxygenase). The recommended dose and its double were tested individually and in combination for this. It should be noted that the protocol used and the initial concentrations selected are the same as those used in the field. After 7 and 14 days (7D/14D) of exposure, all dosages were administered. Our findings show that the pesticides tested had no effect on earthworm survival. However, a significant decrease in their growth rates depending on the different concentrations was observed for the different treatments over the entire exposure period of 7 or 14 D. The greatest reductions (31.62%, 35.04%) are reported after 14D for the high concentrations of Decis alone (D2) as well as for the combined treatment Prosaro/Decis (P2/D2). At the same time, an increase in total protein contents (more than 50% after 14D) as well as a decrease in acetylcholine esterase activity were reported for all treatments. We were also able to identify the induction of oxidative stress after xenobiotic exposure, which is more pronounced at the end of the treatment (14D), resulting in the stimulation of the antioxidant system (gluthione, glutathione S-transférase, catalase) as well as the induction of lipoxygenase, which is responsible for the oxidation of polyunsaturated fatty acids as well as the generation of reactive oxygen species (ROS) involved in the inflammatory phenomenon. Finally, it turns out that the species Aporrectodea caliginosa is sensitive to the different concentrations applied, even those used in the open field, and that Decis (deltamethrin) seems to be more toxic than Prosaro and that the combinaison P2/D2 is as toxic as Decis alone (D2).</p>\",\"PeriodicalId\":11497,\"journal\":{\"name\":\"Ecotoxicology\",\"volume\":\" \",\"pages\":\"1180-1192\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ecotoxicology\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1007/s10646-024-02811-6\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/10/9 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"ECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ecotoxicology","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1007/s10646-024-02811-6","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/9 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"ECOLOGY","Score":null,"Total":0}
Stress biomarker response in Aporrectodea caliginosa earthworms exposed to single and combined pesticide treatments (Prosaro and Decis).
This study aims to assess the impact of two pesticides commonly used in Algeria (Prosaro XRT and Decis 25 EC), as well as their combinations at recommended doses, on a non-target species bioindicator of soil pollution, the earthworm Aporrectodea caliginosa, using physiological (mortality and growth) and biochemical parameters (proteins, glutathione, catalase activity and glutathione S-transferase, acetylcholine esterase, lipoxygenase). The recommended dose and its double were tested individually and in combination for this. It should be noted that the protocol used and the initial concentrations selected are the same as those used in the field. After 7 and 14 days (7D/14D) of exposure, all dosages were administered. Our findings show that the pesticides tested had no effect on earthworm survival. However, a significant decrease in their growth rates depending on the different concentrations was observed for the different treatments over the entire exposure period of 7 or 14 D. The greatest reductions (31.62%, 35.04%) are reported after 14D for the high concentrations of Decis alone (D2) as well as for the combined treatment Prosaro/Decis (P2/D2). At the same time, an increase in total protein contents (more than 50% after 14D) as well as a decrease in acetylcholine esterase activity were reported for all treatments. We were also able to identify the induction of oxidative stress after xenobiotic exposure, which is more pronounced at the end of the treatment (14D), resulting in the stimulation of the antioxidant system (gluthione, glutathione S-transférase, catalase) as well as the induction of lipoxygenase, which is responsible for the oxidation of polyunsaturated fatty acids as well as the generation of reactive oxygen species (ROS) involved in the inflammatory phenomenon. Finally, it turns out that the species Aporrectodea caliginosa is sensitive to the different concentrations applied, even those used in the open field, and that Decis (deltamethrin) seems to be more toxic than Prosaro and that the combinaison P2/D2 is as toxic as Decis alone (D2).
期刊介绍:
Ecotoxicology is an international journal devoted to the publication of fundamental research on the effects of toxic chemicals on populations, communities and terrestrial, freshwater and marine ecosystems. It aims to elucidate mechanisms and processes whereby chemicals exert their effects on ecosystems and the impact caused at the population or community level. The journal is not biased with respect to taxon or biome, and papers that indicate possible new approaches to regulation and control of toxic chemicals and those aiding in formulating ways of conserving threatened species are particularly welcome. Studies on individuals should demonstrate linkage to population effects in clear and quantitative ways. Laboratory studies must show a clear linkage to specific field situations. The journal includes not only original research papers but technical notes and review articles, both invited and submitted. A strong, broadly based editorial board ensures as wide an international coverage as possible.