Aporrectodea caliginosa蚯蚓暴露于单一和混合杀虫剂处理(Prosaro 和 Decis)后的应激生物标志物反应。

IF 2.4 4区 环境科学与生态学 Q2 ECOLOGY
Ecotoxicology Pub Date : 2024-12-01 Epub Date: 2024-10-09 DOI:10.1007/s10646-024-02811-6
Djamila Hafsi, Ibtissem Sbartai, Hana Sbartai
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引用次数: 0

摘要

本研究旨在利用生理(死亡率和生长)和生化参数(蛋白质、谷胱甘肽、过氧化氢酶活性和谷胱甘肽 S-转移酶、乙酰胆碱酯酶、脂氧合酶),评估阿尔及利亚常用的两种杀虫剂(Prosaro XRT 和 Decis 25 EC)及其推荐剂量的组合对土壤污染的非目标物种生物指标蚯蚓 Aporrectodea caliginosa 的影响。为此,对推荐剂量及其双倍剂量进行了单独和联合测试。需要注意的是,所使用的方案和选择的初始浓度与实地使用的相同。经过 7 天和 14 天(7D/14D)的接触后,施用了所有剂量。我们的研究结果表明,测试的杀虫剂对蚯蚓的存活率没有影响。在 14 天后,高浓度的单独癸二酸(D2)以及 Prosaro/Decis 组合处理(P2/D2)的蚯蚓生长率下降幅度最大(分别为 31.62% 和 35.04%)。同时,所有处理的总蛋白质含量都有所增加(14 天后超过 50%),乙酰胆碱酯酶活性也有所下降。我们还发现,在接触异种生物后,氧化应激的诱导作用在处理结束时(14D)更为明显,从而刺激了抗氧化系统(谷胱甘肽、谷胱甘肽 S-转移酶、过氧化氢酶),并诱导了脂氧合酶,该酶负责氧化多不饱和脂肪酸,并产生参与炎症现象的活性氧(ROS)。最后,结果表明,疟原虫(Aporrectodea caliginosa)对不同浓度的杀虫剂很敏感,即使是在野外使用的杀虫剂也是如此,而 Decis(溴氰菊酯)的毒性似乎比 Prosaro 更强,P2/D2 组合的毒性与 Decis 单独使用时(D2)的毒性相同。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Stress biomarker response in Aporrectodea caliginosa earthworms exposed to single and combined pesticide treatments (Prosaro and Decis).

This study aims to assess the impact of two pesticides commonly used in Algeria (Prosaro XRT and Decis 25 EC), as well as their combinations at recommended doses, on a non-target species bioindicator of soil pollution, the earthworm Aporrectodea caliginosa, using physiological (mortality and growth) and biochemical parameters (proteins, glutathione, catalase activity and glutathione S-transferase, acetylcholine esterase, lipoxygenase). The recommended dose and its double were tested individually and in combination for this. It should be noted that the protocol used and the initial concentrations selected are the same as those used in the field. After 7 and 14 days (7D/14D) of exposure, all dosages were administered. Our findings show that the pesticides tested had no effect on earthworm survival. However, a significant decrease in their growth rates depending on the different concentrations was observed for the different treatments over the entire exposure period of 7 or 14 D. The greatest reductions (31.62%, 35.04%) are reported after 14D for the high concentrations of Decis alone (D2) as well as for the combined treatment Prosaro/Decis (P2/D2). At the same time, an increase in total protein contents (more than 50% after 14D) as well as a decrease in acetylcholine esterase activity were reported for all treatments. We were also able to identify the induction of oxidative stress after xenobiotic exposure, which is more pronounced at the end of the treatment (14D), resulting in the stimulation of the antioxidant system (gluthione, glutathione S-transférase, catalase) as well as the induction of lipoxygenase, which is responsible for the oxidation of polyunsaturated fatty acids as well as the generation of reactive oxygen species (ROS) involved in the inflammatory phenomenon. Finally, it turns out that the species Aporrectodea caliginosa is sensitive to the different concentrations applied, even those used in the open field, and that Decis (deltamethrin) seems to be more toxic than Prosaro and that the combinaison P2/D2 is as toxic as Decis alone (D2).

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来源期刊
Ecotoxicology
Ecotoxicology 环境科学-毒理学
CiteScore
5.30
自引率
3.70%
发文量
107
审稿时长
4.7 months
期刊介绍: Ecotoxicology is an international journal devoted to the publication of fundamental research on the effects of toxic chemicals on populations, communities and terrestrial, freshwater and marine ecosystems. It aims to elucidate mechanisms and processes whereby chemicals exert their effects on ecosystems and the impact caused at the population or community level. The journal is not biased with respect to taxon or biome, and papers that indicate possible new approaches to regulation and control of toxic chemicals and those aiding in formulating ways of conserving threatened species are particularly welcome. Studies on individuals should demonstrate linkage to population effects in clear and quantitative ways. Laboratory studies must show a clear linkage to specific field situations. The journal includes not only original research papers but technical notes and review articles, both invited and submitted. A strong, broadly based editorial board ensures as wide an international coverage as possible.
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