Analysis of synthetic route and case correlation of methamphetamine crystals seized by ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) and Pearson correlation coefficient method†

Analysis of impurities in illicit drugs provides key information on the manufacture and distribution of methamphetamine (MA). An analysis of 22 specific target compounds in unlawful MA products was conducted using ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS). The peak area of the alkaloids was utilized as an important calculation data variable to determine the synthesis method. The results demonstrated that the application of target screening techniques for trace impurity components in samples can aid in determining the synthesis process routes for toxin production in 169 samples. These samples were initially categorized based on their synthesis modes, specifically synthesis routes N, E, and R. The correlation analysis of MA samples with known synthetic routes was carried out using principal coordinate analysis (PCoA) and the Pearson correlation coefficient method. Among the N and R synthesis routes, 15 and 7 groups with strong correlation were screened, respectively. Consistent with the observed scenario, the samples from the synthetic route E showed limited correlation with each other. The experiment provides a focused framework for the management of synthetic raw materials used for the production of drugs and the investigation of drug-related cases. This facilitates a holistic strategy to combat drug-related crimes, from manufacturing laboratory to end-user.